鉴定 chalkbrood 病病原体 Ascospheara apis 中的长非编码 RNA。

Identification of long non-coding RNAs in the chalkbrood disease pathogen Ascospheara apis.

机构信息

College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

出版信息

J Invertebr Pathol. 2018 Jul;156:1-5. doi: 10.1016/j.jip.2018.06.001. Epub 2018 Jun 9.

DOI:10.1016/j.jip.2018.06.001

Abstract

Ascospheara apis is a widespread fungal pathogen that exclusively invades honeybee larvae. Thus far, non-coding RNA in A. apis has not yet been documented. In this study, we sequenced A. apis using strand specific cDNA library construction and Illumina RNA sequencing methods, and identified 379 lncRNAs, including antisense lncRNAs, lincRNAs, intronic lncRNAs and sense lncRNAs. Additionally, these lncRNAs were found to be shorter in length and have fewer exons and transcript isoforms than protein-coding genes, similar to those identified in mammals and plants. Furthermore, the existence of 15 predicted lncRNAs of A. apis was confirmed using RT-PCR and expression levels of 11 were lower than those of adjacent protein-coding genes. Our findings not only enlarge the lncRNA database for fungi, but also lay a foundation for further investigation of potential lncRNA-mediated regulation of genes in A. apis.

摘要

蜜粉囊腔菌是一种广泛存在的真菌病原体，专门侵袭蜜蜂幼虫。迄今为止，尚未有关于蜜粉囊腔菌非编码 RNA 的记载。在这项研究中，我们使用链特异性 cDNA 文库构建和 Illumina RNA 测序方法对蜜粉囊腔菌进行了测序，鉴定出 379 个 lncRNA，包括反义 lncRNA、lincRNA、内含子 lncRNA 和正义 lncRNA。此外，这些 lncRNA 的长度更短，外显子和转录本异构体比蛋白质编码基因更少，与在哺乳动物和植物中鉴定到的 lncRNA 相似。此外，通过 RT-PCR 验证了 15 个预测的蜜粉囊腔菌 lncRNA 的存在，其中 11 个的表达水平低于相邻的蛋白质编码基因。我们的研究结果不仅扩大了真菌的 lncRNA 数据库，还为进一步研究蜜粉囊腔菌中潜在的 lncRNA 介导的基因调控奠定了基础。