Department of Inorganic and Analytical Chemistry, Faculty of Chemistry, University of Lodz, Tamka 12, 91-403 Lodz, Poland.
Department of Molecular Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-237 Lodz, Poland.
Bioelectrochemistry. 2018 Oct;123:227-232. doi: 10.1016/j.bioelechem.2018.06.002. Epub 2018 Jun 2.
In the present study, the electrochemical behavior of the antiviral drug tenofovir (tnf) on a boron-doped diamond electrode (BDDE) has been studied using square-wave voltammetry (SWV). The experimental conditions such as the supporting electrolyte composition and the SWV parameters were optimized. Under the optimized conditions, a simple and sensitive SWV procedure for tnf determination was developed in the concentration range of 5.0 × 10 to 1.0 × 10 mol L. The calculated limit of detection and limit of quantification were equal to 5.6 × 10 and 1.9 × 10 mol L, respectively. The biological significance of the developed method was demonstrated by a quantitative analysis of the pharmaceutical formulations Viread and Tenofovir disoproxil Teva. Moreover, both voltammetric and spectroscopic techniques were used to study the interaction between tnf and DNA. Changes in the electrochemical and spectroscopic behavior of tnf in the presence of DNA were used to calculate the binding constants of the formed complexes. The estimated values of Gibbs free energy revealed that the formation of the drug-DNA complexes was a spontaneous and favorable process. Moreover, for free and bound tenofovir, kinetic parameters such as heterogeneous rate constant, electron transfer coefficient, and diffusion coefficient were determined.
在本研究中，使用方波伏安法（SWV）研究了抗病毒药物替诺福韦（tnf）在掺硼金刚石电极（BDDE）上的电化学行为。优化了实验条件，如支持电解质的组成和 SWV 参数。在优化条件下，开发了一种简单灵敏的 SWV 程序，用于在 5.0×10 至 1.0×10 mol/L 的浓度范围内测定 tnf。计算的检出限和定量限分别等于 5.6×10 和 1.9×10 mol/L。通过对 Viread 和替诺福韦酯泰华的药物制剂进行定量分析，证明了所开发方法的生物学意义。此外，还使用伏安法和光谱技术研究了 tnf 与 DNA 之间的相互作用。tnf 在存在 DNA 时电化学和光谱行为的变化用于计算形成的配合物的结合常数。估计的吉布斯自由能值表明，药物-DNA 配合物的形成是一个自发和有利的过程。此外，还测定了游离和结合的替诺福韦的动力学参数，如不均匀速率常数、电子转移系数和扩散系数。
