Krause Daniela, Stapf Theresa M, Kirnich Verena B, Hennings Anika, Riemer Sabine, Chrobok Agnieszka, Fries Daniel R, Pedrosa Gil Francisco, Rief Winfried, Schwarz Markus J, Schmidmaier Ralf
Department of Psychiatry and Psychotherapy, University Hospital, LMU Munich, Munich, Germany.
Division of Clinical Psychology and Psychotherapy, Philipps University of Marburg, Marburg, Germany.
Neuroimmunomodulation. 2018;25(1):7-17. doi: 10.1159/000488353. Epub 2018 Jun 12.
Cellular immune status in major depression (MD) patients differs from that in somatoform disorder (SFD) patients and healthy controls (HC). It is still questionable whether the patterns of immune parameters remain stable over time. Therefore, we studied lymphocyte and monocyte cell counts and neopterin levels in peripheral blood of MD and SFD patients and HC over 12 weeks and tested for correlations between biochemical and psychometric parameters.
Thirty-nine patients with MD, 27 with SFD, and 51 HC were recruited. Peripheral blood was drawn at four visits, at 4-week intervals. We assessed the total cell count of B lymphocytes, natural killer (NK) cells, T lymphocyte subpopu-lations, and monocytes by flow cytometry, and neopterin serum levels by ELISA. Psychometric parameters were measured with questionnaires.
Counts of lymphocytes, monocytes, and neopterin were stable in the SFD and HC groups. In the MD group, total CD3+, CD3+CD8+, NK cells, and CD3+CD25+ T cells showed inhomogeneous variances in Friedman tests, particularly in females. Neopterin correlated with depressed mood in MD patients, and with body mass index in HC.
Cellular immune parameters are stable in HC and SFD. Our results may indicate influences of MD and gender on some cellular immune parameters. This may need to be considered in future immunological studies.
重度抑郁症（MD）患者的细胞免疫状态与躯体形式障碍（SFD）患者及健康对照（HC）不同。免疫参数模式随时间是否保持稳定仍存在疑问。因此，我们研究了MD和SFD患者以及HC外周血中淋巴细胞和单核细胞计数及新蝶呤水平，为期12周，并检测了生化参数与心理测量参数之间的相关性。
招募了39例MD患者、27例SFD患者和51例HC。每隔4周进行4次外周血采集。我们通过流式细胞术评估B淋巴细胞、自然杀伤（NK）细胞、T淋巴细胞亚群和单核细胞的细胞总数，通过酶联免疫吸附测定法评估血清新蝶呤水平。用问卷测量心理测量参数。
SFD组和HC组的淋巴细胞、单核细胞计数及新蝶呤水平稳定。在MD组中，Friedman检验显示总CD3 +、CD3 + CD8 +、NK细胞和CD3 + CD25 + T细胞存在不均匀方差，尤其是在女性中。MD患者的新蝶呤与抑郁情绪相关，HC中的新蝶呤与体重指数相关。
HC和SFD中的细胞免疫参数稳定。我们的结果可能表明MD和性别对某些细胞免疫参数有影响。这可能需要在未来的免疫学研究中加以考虑。
