School of Chemistry, The University of New South Wales, Sydney, NSW, 2052, Australia.
Australian Centre for NanoMedicine, The University of New South Wales, Sydney, NSW, 2052, Australia.
Nat Commun. 2018 Jun 12;9(1):2288. doi: 10.1038/s41467-018-04701-y.
For many normal and aberrant cell behaviours, it is important to understand the origin of cellular heterogeneity. Although powerful methods for studying cell heterogeneity have emerged, they are more suitable for common rather than rare cells. Exploring the heterogeneity of rare single cells is challenging because these rare cells must be first pre-concentrated and undergo analysis prior to classification and expansion. Here, a versatile capture & release platform consisting of an antibody-modified and electrochemically cleavable semiconducting silicon surface for release of individual cells of interest is presented. The captured cells can be interrogated microscopically and tested for drug responsiveness prior to release and recovery. The capture & release strategy was applied to identify rare tumour cells from whole blood, monitor the uptake of, and response to, doxorubicin and subsequently select cells for single-cell gene expression based on their response to the doxorubicin.
对于许多正常和异常的细胞行为，了解细胞异质性的起源非常重要。尽管已经出现了用于研究细胞异质性的强大方法，但它们更适合于常见细胞而不是罕见细胞。探索罕见单细胞的异质性具有挑战性，因为这些罕见细胞必须首先进行预浓缩，然后才能进行分类和扩增分析。在这里，提出了一种由抗体修饰和电化学可裂解的半导体硅表面组成的通用捕获和释放平台，用于释放感兴趣的单个细胞。可以在释放和回收之前对捕获的细胞进行微观检测，并测试其对药物的反应性。该捕获和释放策略用于从全血中鉴定罕见的肿瘤细胞，监测阿霉素的摄取和反应，然后根据阿霉素的反应选择单细胞基因表达的细胞。
