青蛙胚胎试验中诱导的致畸表型的组织学观察。
Histological Observation of Teratogenic Phenotypes Induced in Frog Embryo Assays.
作者信息
Pownall Mark E, Saha Margaret S
机构信息
Department of Biology, College of William and Mary, Williamsburg, VA, USA.
出版信息
Methods Mol Biol. 2018;1797:309-323. doi: 10.1007/978-1-4939-7883-0_16.
Abstract
Amphibian embryos have long served as an ideal model for teratogenicity testing. While whole-mount embryo observations can be utilized, histological observation of teratogenic phenotypes provides a wealth of additional information that can lead to mechanistic insights. In this chapter, detailed protocols for two methods of sectioning embryos as well as a guide for histological analysis is provided.
摘要
两栖动物胚胎长期以来一直是致畸性测试的理想模型。虽然可以利用整体胚胎观察，但对致畸表型进行组织学观察可提供大量额外信息，从而有助于深入了解其作用机制。在本章中，我们提供了两种胚胎切片方法的详细方案以及组织学分析指南。
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