Chun Honggu
Department of Biomedical Engineering, Korea University, Seongbukgu, Seoul, Korea.
Electrophoresis. 2018 Sep;39(17):2181-2187. doi: 10.1002/elps.201800093. Epub 2018 Jun 28.
A nanoporous poly-(styrene sulfonate) (poly-SS) membrane was developed for fast and selective ion transport in a microfluidic chip. The poly-SS membrane can be photopolymerized in-situ at arbitrary location of a microchannel, enabling integrated fluidics design in the microfluidic chip. The membrane is characterized by a low hydraulic resistance and a high surface charge to maximize the electroosmotic flow and charge selectivity. The membrane characteristics were investigated by charge-selective electropreconcentration method. Experimental results show membranes with various percentages of poly-SS are able to concentrate anions (fluorescein and TRITC-labeled BSA). The anion-selective electropreconcentration process is stable and 26-times faster than previously reported poly-AMPS (2-acrylamido-2-methyl-1-propanesulfonic acid) based system. The electropreconcentration was also demonstrated to depend on the sample valency and buffer concentration.
开发了一种纳米多孔聚（苯乙烯磺酸盐）（聚-SS）膜，用于微流控芯片中的快速选择性离子传输。聚-SS膜可在微通道的任意位置原位光聚合，从而实现微流控芯片中的集成流体设计。该膜具有低水力阻力和高表面电荷的特点，可使电渗流和电荷选择性最大化。通过电荷选择性电预浓缩方法研究了膜的特性。实验结果表明，具有不同聚-SS百分比的膜能够浓缩阴离子（荧光素和TRITC标记的牛血清白蛋白）。阴离子选择性电预浓缩过程稳定，比先前报道的基于聚-AMPS（2-丙烯酰胺基-2-甲基-1-丙烷磺酸）的系统快26倍。还证明了电预浓缩取决于样品价态和缓冲液浓度。
