Department of Chemistry, University of North Carolina at Chapel Hill, Chapman Hall, CB#3216, Chapel Hill, North Carolina 27599, USA.
Anal Chem. 2010 Jul 15;82(14):6287-92. doi: 10.1021/ac101297t.
The development and analysis of a microfluidic sample preconcentration system using a highly ion-conductive charge-selective polymer [poly-AMPS (2-acrylamido-2-methyl-1-propanesulfonic acid)] is reported. The preconcentration is based on the phenomenon of concentration polarization which develops at the boundaries of the poly-AMPS with buffer solutions. A negatively charged polymer, poly-AMPS, positioned between two microchannels efficiently extracts cations through its large cross section, resulting in efficient anion sample preconcentration. The present work includes the development of a robust polymer that is stable over a wide range of buffers with varying chemical compositions. The sample preconcentration effect remains linear to over 3 mM (0.15 pmol) and 500 microM (15 fmol) for fluorescein and TRITC-tagged albumin solutions, respectively. The system can potentially be used for concentrating proteins on microfluidic devices with subsequent analysis for proteomic applications.
本文报道了一种使用高导电性荷选择聚合物[聚 AMPS(2-丙烯酰胺基-2-甲基-1-丙磺酸)]开发和分析微流控样品预浓缩系统的方法。该预浓缩基于在聚 AMPS 与缓冲溶液的边界处发生的浓差极化现象。带负电荷的聚合物聚 AMPS 位于两个微通道之间，通过其大横截面有效地提取阳离子，从而实现有效的阴离子样品预浓缩。本工作包括开发一种在具有不同化学成分的各种缓冲液中稳定的坚固聚合物。对于荧光素和 TRITC 标记的白蛋白溶液，样品预浓缩效果分别在超过 3 mM(0.15 pmol)和 500 microM(15 fmol)时仍保持线性。该系统可用于在微流控设备上浓缩蛋白质，随后用于蛋白质组学应用的分析。
