Chen Wei, Zhao Wei, Chen Sheng, Zhang Li, Guo Zhongying, Wang Lixin, Wang Jipeng, Wan Zongren, Hong Yongqing, Yu Liang
Department of Respiratory Medicine, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu, China.
Department of Pathology, The Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Clin Respir J. 2018 Jul;12(7):2284-2291. doi: 10.1111/crj.12906. Epub 2018 Jun 13.
Non-small cell lung cancer (NSCLC) is the most common cause of cancer-related deaths in the world. MALAT1 and SOX9 have important roles in tumour formation and development in several types of cancers. However, little is known about the function and co-relationship of these 2 factors in NSCLC in vivo.
To explore the role of MALAT1 and SOX9 expression relationship, their clinical pathological characteristics and OS on NSCLC patients.
Paired of primary lung cancer tissues and the matched tumour adjacent tissues were collected in 121 NSCLC patients. MALAT1 and SOX9 mRNA expression was measured by SYBR green q RT-PCR assay. SOX-9 protein expression was measured by streptavidin-peroxidase (SP) staining method.
MALAT1and SOX9 expression was higher in NSCLC tissues than the adjacent tissues, and they have positive correlation. Moreover, SOX9 protein expression was higher in NSCLC tissues, especially in MALAT1 mRNA higher expressed NSCLC tissues. MALAT1 and SOX9 mRNA expression were associated with age (x =11.474, P = .009), tumour size (x =26.839, P = .000), TNM stage (x =8.010, P = .046) and LEL. (x =53.908, P = .000). NSCLC patients with higher MALAT1 and SOX9 mRNA expression had poorer OS rates.
MALAT1 and SOX9 could be used as prognostic co-biomarker in NSCLC.
非小细胞肺癌（NSCLC）是全球癌症相关死亡的最常见原因。MALAT1和SOX9在多种癌症的肿瘤形成和发展中发挥重要作用。然而，关于这两个因子在NSCLC体内的功能及相互关系知之甚少。
探讨MALAT1和SOX9的表达关系、临床病理特征及其对NSCLC患者总生存期（OS）的影响。
收集121例NSCLC患者的配对原发性肺癌组织及相应的肿瘤旁组织。采用SYBR green q RT-PCR法检测MALAT1和SOX9 mRNA表达。采用链霉亲和素-过氧化物酶（SP）染色法检测SOX-9蛋白表达。
NSCLC组织中MALAT1和SOX9的表达高于癌旁组织，且二者呈正相关。此外，NSCLC组织中SOX9蛋白表达较高，尤其是在MALAT1 mRNA高表达的NSCLC组织中。MALAT1和SOX9 mRNA表达与年龄（χ=11.474，P=0.009）、肿瘤大小（χ=26.839，P=0.000）、TNM分期（χ=8.010，P=0.046）及淋巴细胞浸润（χ=53.908，P=0.000）有关。MALAT1和SOX9 mRNA表达较高的NSCLC患者的OS率较差。
MALAT1和SOX9可作为NSCLC的预后联合生物标志物。
