Zheng Chen-na, Duan Xun-wei, Jia Dong-fang, Luo Cai-lin, Wang Ning-qing, Yang Hui-yong, Diao Yong
Yao Xue Xue Bao. 2016 Jul;51(7):1077-82.
This study was designed to evaluate the anti-inflammatory effect of recombinant human kallistatin (Kal) on ulcerative colitis (UC) in the mouse model. Acute colitis was induced by administration of 4% dextran sodium suffate (DSS) to KM mice for 7 days. The mice were then randomized into 5 groups: model control, Kal 0.2 mg·kg(-1)·d(-1), 1.0 mg·kg(-1)·d(-1) and 2.0 mg·kg-1·d(-1) group, salazosulfapyridine (SASP) group. Ten age-matched normal KM mouse were administered with saline in the normal control. The weight, colon length, inflammation factor (MPO/SOD/MDA) and TNF-α/IL-10 levels among the five groups of mice were determined. The results showed that histological index score and MPO/MDA/TNF-α levels of high-dose Kal treatment group and SASP group were significantly lower compared with the model group (P < 0.01), but the weight, colon length, IL-10 level and SOD activity were significant higher than the model group (P < 0.01), approaching the normal group. These parameters showed that Kal can significantly relieve the UC state in a dose-dependent manner. This study demonstrates that Kal significantly remits UC in mice, and participates in the regulation of inflammatory cytokines TNF-α/IL-10 levels and has some antioxidant activity.
本研究旨在评估重组人组织激肽释放酶抑制因子（Kal）对小鼠溃疡性结肠炎（UC）模型的抗炎作用。通过给KM小鼠饮用4%葡聚糖硫酸钠（DSS）7天诱导急性结肠炎。然后将小鼠随机分为5组：模型对照组、Kal 0.2 mg·kg(-1)·d(-1)组、1.0 mg·kg(-1)·d(-1)组、2.0 mg·kg(-1)·d(-1)组、柳氮磺胺吡啶（SASP）组。10只年龄匹配的正常KM小鼠作为正常对照组给予生理盐水。测定五组小鼠的体重、结肠长度、炎症因子（MPO/SOD/MDA）以及TNF-α/IL-10水平。结果显示，高剂量Kal治疗组和SASP组的组织学指数评分以及MPO/MDA/TNF-α水平显著低于模型组（P < 0.01），但体重、结肠长度、IL-10水平和SOD活性显著高于模型组（P < 0.01），接近正常组。这些参数表明Kal能够以剂量依赖的方式显著缓解UC状态。本研究表明Kal能显著减轻小鼠的UC症状，并参与调节炎性细胞因子TNF-α/IL-10水平，且具有一定的抗氧化活性。
