Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Int Wound J. 2018 Oct;15(5):807-813. doi: 10.1111/iwj.12931. Epub 2018 Jun 13.
The aim of this study was to compare protein secretion on intact skin of extremities and verify the relationship between the marker proteins on abdominal skin and systemic factors using skin blotting. A cross-sectional study was conducted among elderly patients aged 65 years and older (N = 73) at a long-term medical facility in Japan. Skin blotting was performed on the right and left forearms, right and left lower legs, and abdomen. Pearson's correlations and Bland-Altman plots were utilised for comparing the protein secretion from the skin between the right or left forearms or lower legs. Multiple regression analysis was applied to determine the relationship between intensity levels of 3 proteins on the abdominal skin and the systemic factors. Bland-Altman plots demonstrated that there was no significant difference between right and left secretion levels on the forearms and lower legs among 3 proteins. Multiple regression analysis showed that age and antiplatelet use was positively associated with decreased collagen type IV and increased matrix metalloproteinase 2 levels, respectively. Our findings suggested that collecting samples from either the right or the left skin would be sufficient if skin properties between arms and legs are evaluated using skin blotting.
本研究旨在比较四肢完整皮肤的蛋白质分泌情况，并使用皮肤印迹法验证腹部皮肤标志物蛋白与全身因素之间的关系。本研究在日本一家长期医疗机构中进行了一项横断面研究，纳入了 73 名年龄在 65 岁及以上的老年患者。在右前臂、左前臂、右小腿、左小腿和腹部进行了皮肤印迹法。利用 Pearson 相关分析和 Bland-Altman 图比较了右前臂或左前臂或小腿皮肤之间的蛋白质分泌情况。应用多元回归分析确定了腹部皮肤 3 种蛋白质强度水平与全身因素之间的关系。Bland-Altman 图表明，在 3 种蛋白质中，右前臂和左前臂以及右小腿和左小腿之间的分泌水平没有显著差异。多元回归分析显示，年龄和抗血小板药物的使用与Ⅳ型胶原水平降低和基质金属蛋白酶 2 水平升高分别呈正相关。我们的研究结果表明，如果使用皮肤印迹法评估手臂和腿部皮肤的特性，那么从右侧或左侧皮肤采集样本就足够了。
