Minematsu Takeo, Horii Motoko, Oe Makoto, Sugama Junko, Mugita Yuko, Huang Lijuan, Nakagami Gojiro, Sanada Hiromi
Takeo Minematsu, PhD, is a Project Lecturer, Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Japan. Motoko Horii, MHS, is an Assistant Professor, Faculty of Nursing, Josai International University, Chiba, Japan. Makoto Oe, PhD, is a Project Lecturer, Department of Advanced Nursing Technology, Graduate School of Medicine, The University of Tokyo. Junko Sugama, PhD, is a Professor, Department of Clinical Nursing, Graduate School of Medical Science, Kanazawa University, Ishikawa, Japan. Yuko Mugita, MHS, is a Doctoral Student; Lijuan Huang, PhD, is a Project Researcher; Gojiro Nakagami, PhD, is a Lecturer; and Hiromi Sanada, PhD, is a Professor, all at the Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Japan.
Adv Skin Wound Care. 2014 Jun;27(6):272-9. doi: 10.1097/01.ASW.0000448461.25542.36.
The skin performs important structural and physiological functions, and skin assessment represents an important step in identifying skin problems. Although noninvasive techniques for assessing skin status exist, no such techniques for monitoring its physiological status are available. This study aimed to develop a novel skin-assessment technique known as skin blotting, based on the leakage of secreted proteins from inside the skin following overhydration in mice. The applicability of this technique was further investigated in a clinical setting.
Skin blotting involves 2 steps: collecting proteins by attaching a damp nitrocellulose membrane to the surface of the skin, and immunostaining the collected proteins. The authors implanted fluorescein-conjugated dextran (F-DEX)-containing agarose gels into mice and detected the tissue distribution of F-DEX under different blotting conditions. They also analyzed the correlations between inflammatory cytokine secretion and leakage following ultraviolet irradiation in mice and in relation to body mass index in humans.
The F-DEX in mice was distributed in the deeper and shallower layers of skin and leaked through the transfollicular and transepidermal routes, respectively. Ultraviolet irradiation induced tumor necrosis factor secretion in the epidermis in mice, which was detected by skin blotting, whereas follicular tumor necrosis factor was associated with body mass index in obese human subjects. These results support the applicability of skin blotting for skin assessment.
Skin blotting represents a noninvasive technique for assessing skin physiology and has potential as a predictive and diagnostic tool for skin disorders.
皮肤具有重要的结构和生理功能，皮肤评估是识别皮肤问题的重要步骤。虽然存在评估皮肤状态的非侵入性技术，但尚无监测其生理状态的此类技术。本研究旨在基于小鼠过度水化后皮肤内分泌蛋白的泄漏，开发一种名为皮肤印迹法的新型皮肤评估技术。并在临床环境中进一步研究了该技术的适用性。
皮肤印迹法包括两个步骤：通过将潮湿的硝酸纤维素膜附着在皮肤表面收集蛋白质，以及对收集的蛋白质进行免疫染色。作者将含有荧光素偶联葡聚糖（F-DEX）的琼脂糖凝胶植入小鼠体内，并在不同的印迹条件下检测F-DEX的组织分布。他们还分析了小鼠紫外线照射后炎症细胞因子分泌与泄漏之间的相关性，以及与人类体重指数的关系。
小鼠体内的F-DEX分别分布在皮肤的深层和浅层，并分别通过毛囊和表皮途径泄漏。紫外线照射诱导小鼠表皮肿瘤坏死因子分泌，可通过皮肤印迹法检测到，而毛囊肿瘤坏死因子与肥胖人类受试者的体重指数相关。这些结果支持皮肤印迹法在皮肤评估中的适用性。
皮肤印迹法是一种评估皮肤生理功能的非侵入性技术，具有作为皮肤疾病预测和诊断工具的潜力。
