Vázquez F, Palacios S, Alemañ N, Guerrero F
Department of Gynaecology and Obstetrics, Ceoga, Lugo, Spain.
Maturitas. 1996 Nov;25(3):209-15. doi: 10.1016/s0378-5122(96)01066-3.
To determine the relationship between skin collagen IV and basement membrane changes during aging, a total of 35 women who had been admitted for surgery, were studied.
Subjects were arranged into six age-groups (from 35 to 60 years). Skin biopsies were performed in all patients and the samples were taken from a site 6 cm above the pubic symphysis. The collagen IV content and the epithelial basement membrane were analyzed by using immunohistochemical, transmission electron microscopy and computer-assisted image analysis methods. The skin collagen IV content was measured by an image analysis program and expressed in arbitrary units of integrate optical density, and, the basement membrane thickness was expressed in nanometers.
Type IV collagen content decreased with age after 35 years (r = -0.9561). The epithelial basement membrane thickness increased significantly with age (r = 0.98192; P < 0.01) and there is an inverse correlation between these two parameters (r = -0.990502).
Although type IV collagen is a basement membrane component and declines with aging, the total thickness of this membrane increases, which suggests a reduction in tissue turnover.
为确定衰老过程中皮肤IV型胶原蛋白与基底膜变化之间的关系，对35例接受手术的女性进行了研究。
将受试者分为六个年龄组（35至60岁）。对所有患者进行皮肤活检，样本取自耻骨联合上方6厘米处。采用免疫组织化学、透射电子显微镜和计算机辅助图像分析方法分析IV型胶原蛋白含量和上皮基底膜。通过图像分析程序测量皮肤IV型胶原蛋白含量，以积分光密度的任意单位表示，基底膜厚度以纳米表示。
35岁以后，IV型胶原蛋白含量随年龄增长而下降（r = -0.9561）。上皮基底膜厚度随年龄增长显著增加（r = 0.98192；P < 0.01），且这两个参数之间存在负相关（r = -0.990502）。
虽然IV型胶原蛋白是基底膜的组成成分且随衰老而减少，但该膜的总厚度增加，这表明组织更新减少。
