South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, P. R. China.
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Guangzhou, P. R. China.
PLoS One. 2018 Jun 13;13(6):e0199053. doi: 10.1371/journal.pone.0199053. eCollection 2018.
Sthenoteuthis oualaniensis (SA) is an important squid species in the South China Sea. Based on SA samples collected in 2016, SA was divided into the "dwarf" form (DF) and "medium" form (MF). To understand the changes in gastrointestinal function in SA during sexual maturation, we undertook transcriptomic analyses of the stomach and intestine tissues of the mature and immature DF and MF of SA using the deep-sequencing platform Illumina HiSeq™. We exploited a high-throughput method to delineate differentially expressed genes (DEGs) in the DF and MF of SA. A total of 135464 unigenes (68627 unigenes of the DG and 66837 unigenes of the MF) were generated. We identified 7965 and 4051 relative DEGs in the intestine and stomach tissues of the mature DF of SA compared with those of the immature DF of SA; and 22138 and 18460 DEGs in the intestine and stomach of the mature MF of SA compared with those of the immature MF of SA. Gastrointestinal function related to the metabolism of lipids, amino acids, glucose, and energy were changed in SA during sexual maturation. This work is the first to identify a set of genes associated with gastrointestinal function during sexual maturation in SA.
新月枪形多柔鱼（Sthenoteuthis oualaniensis）是南海的一种重要鱿鱼物种。基于 2016 年采集的新月枪形多柔鱼样本，将其分为“矮型”（DF）和“中型”（MF）两种形态。为了了解性成熟过程中新月枪形多柔鱼胃肠道功能的变化，我们利用 Illumina HiSeq™高通量测序平台对成熟和未成熟 DF 和 MF 的胃和肠组织进行了转录组分析。我们采用高通量方法对 SA 的 DF 和 MF 中的差异表达基因（DEGs）进行了描绘。共生成了 135464 个 unigenes（68627 个 DG unigenes和 66837 个 MF unigenes）。我们在成熟 DF 的肠和胃组织中分别鉴定到了 7965 个和 4051 个相对 DEGs，而在成熟 MF 的肠和胃组织中分别鉴定到了 22138 个和 18460 个 DEGs，与未成熟的相比。在性成熟过程中，SA 的脂质、氨基酸、葡萄糖和能量代谢相关的胃肠道功能发生了变化。这项工作首次鉴定出了与 SA 性成熟过程中胃肠道功能相关的一组基因。
