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稀有鮈鲫大脑性别差异基因表达的转录组分析及三丁基锡暴露的影响

Transcriptome analyses of sex differential gene expression in brains of rare minnow () and effects of tributyltin exposure.

作者信息

Zhang Ji-Liang, Zhang Chun-Nuan, Liu Min, Fan Ming-Zhen, Huang Mao-Xian

机构信息

Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, 263 Kaiyuan Road, Luoyang 471023, Henan, China.

出版信息

Data Brief. 2018 Mar 29;18:1193-1195. doi: 10.1016/j.dib.2018.03.119. eCollection 2018 Jun.

DOI:10.1016/j.dib.2018.03.119
PMID:29900293
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5996493/
Abstract

RNA-sequencing was used to identify sex-biased gene expression in brains of rare minnow () by comparing transcriptomic profiles between females and males. Furthermore, transcriptomic responses to 10 ng/L tributyltin (TBT) in both male and female brains were also investigated to understand whether TBT affects the identified sex-biased genes. Differentially expressed genes (DEGs) were identified using the IDEG6 web tool. In this article, we presented male- and female-biased DEGs, and up-regulated and down-regulated DEGs after TBT exposure. The raw reads data supporting the present analyses has been deposited in NCBI Sequence Read Archive (SRA, http://www.ncbi.nlm.nih.gov/Traces/sra) with accession number PRJNA376634. The data presented in this article are related to the research article entitled "Transcriptomic analyses of sexual dimorphism of rare minnow () brains and effects of tributyltin exposure" (doi: 10.1016/j.ecoenv.2018.02.049).

摘要

通过比较稀有鮈鲫雌雄个体的转录组图谱,利用RNA测序来鉴定其大脑中性别偏向性基因表达。此外,还研究了雌雄大脑对10纳克/升三丁基锡(TBT) 的转录组反应,以了解TBT是否会影响已鉴定出的性别偏向性基因。使用IDEG6网络工具鉴定差异表达基因(DEG)。在本文中,我们展示了雄性和雌性偏向性DEG,以及TBT暴露后上调和下调的DEG。支持本分析的原始读数数据已存入NCBI序列读数存档库(SRA,http://www.ncbi.nlm.nih.gov/Traces/sra),登录号为PRJNA376634。本文呈现的数据与题为《稀有鮈鲫大脑性二态性的转录组分析及三丁基锡暴露的影响》的研究论文相关(doi: 10.1016/j.ecoenv.2018.02.049)。

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本文引用的文献

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Transcriptomic analyses of tributyltin-induced sexual dimorphisms in rare minnow (Gobiocypris rarus) brains.三丁基锡诱导稀有鲫脑部性别二态性的转录组分析。
Ecotoxicol Environ Saf. 2018 Jul 30;156:18-24. doi: 10.1016/j.ecoenv.2018.02.049. Epub 2018 Mar 7.
2
SNP identification by transcriptome sequencing and candidate gene-based association analysis for heat tolerance in the bay scallop Argopecten irradians.通过转录组测序鉴定海湾扇贝(Argopecten irradians)耐热性的单核苷酸多态性及基于候选基因的关联分析
PLoS One. 2014 Aug 14;9(8):e104960. doi: 10.1371/journal.pone.0104960. eCollection 2014.
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Full-length transcriptome assembly from RNA-Seq data without a reference genome.无参考基因组的 RNA-Seq 数据的全长转录组组装。
Nat Biotechnol. 2011 May 15;29(7):644-52. doi: 10.1038/nbt.1883.
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Mapping and quantifying mammalian transcriptomes by RNA-Seq.通过RNA测序对哺乳动物转录组进行定位和定量分析。
Nat Methods. 2008 Jul;5(7):621-8. doi: 10.1038/nmeth.1226. Epub 2008 May 30.
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IDEG6: a web tool for detection of differentially expressed genes in multiple tag sampling experiments.IDEG6:一种用于在多重标签采样实验中检测差异表达基因的网络工具。
Physiol Genomics. 2003 Jan 15;12(2):159-62. doi: 10.1152/physiolgenomics.00096.2002.