Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Henan, China.
Laboratory of Aquatic Environment and Animal Safety, College of Animal Science and Technology, Henan University of Science and Technology, Henan, China.
Fish Shellfish Immunol. 2017 Dec;71:10-18. doi: 10.1016/j.fsi.2017.09.066. Epub 2017 Sep 28.
Tributyltin (TBT) is reported to induce adipogenesis in fish, which might affect nutritional qualities and health status. Muscle tissues account for the majority of body mass, and have been described as a major site of fat deposition and an immunologically active organ. Therefore, the present study aims to evaluate whether chronic exposures of TBT, at environmental concentrations of 1, 10 and 100 ng/L, affects lipid accumulation, oxidative stress and immune status in muscle tissues of rare minnow (Gobiocypris rarus). After 60 d of exposure, TBT increased contents of total lipid, total cholesterol, triglyceride and fatty acids in muscle tissues. Interestingly, TBT exposure disrupted fatty acid composition and increased contents of unsaturated fatty acids (such as eicosapentaenoic acid and docosahexaenoic acid) in muscle tissues, which might be a response to preserve membrane functions from TBT exposure. Meanwhile, the concentrations of hepatic fatty acid desaturase 2 (Δ6-desaturase) and stearoyl-CoA desaturase (Δ9-desaturase) were increased after TBT exposure, which might contribute the increase of unsaturated fatty acids. Furthermore, TBT increased muscle lipid peroxidation products, antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), and the expression of immune-related molecules (tumor necrosis factor alpha, interleukin 1 beta and nuclear factor kappa B) in muscle tissues. The disruption of TBT on the lipid accumulation, oxidative stress and immune-toxic effects in muscle tissues of fish might reduce nutritional qualities, and affect growth and health status, which might pose a constant and serious threat to fish and result in economic loss in aquaculture.
三丁基锡（TBT）据报道会在鱼类中诱导脂肪生成，这可能会影响其营养价值和健康状况。肌肉组织占身体质量的大部分，被描述为脂肪沉积的主要部位和具有免疫活性的器官。因此，本研究旨在评估环境浓度为 1、10 和 100ng/L 的 TBT 慢性暴露是否会影响稀有鮈鲫（Gobiocypris rarus）肌肉组织中的脂肪积累、氧化应激和免疫状态。暴露 60 天后，TBT 增加了肌肉组织中的总脂质、总胆固醇、甘油三酯和脂肪酸含量。有趣的是，TBT 暴露破坏了脂肪酸组成，并增加了肌肉组织中不饱和脂肪酸（如二十碳五烯酸和二十二碳六烯酸）的含量，这可能是为了保护膜功能免受 TBT 暴露的影响。同时，暴露于 TBT 后，肝脏脂肪酸去饱和酶 2（Δ6-去饱和酶）和硬脂酰辅酶 A 去饱和酶（Δ9-去饱和酶）的浓度增加，这可能有助于增加不饱和脂肪酸的含量。此外，TBT 增加了肌肉组织中的脂质过氧化产物、抗氧化酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）以及免疫相关分子（肿瘤坏死因子-α、白细胞介素 1β 和核因子-κB）的表达。TBT 对鱼类肌肉组织中脂肪积累、氧化应激和免疫毒性的破坏可能会降低其营养价值，并影响其生长和健康状况，这可能对鱼类构成持续且严重的威胁，并导致水产养殖的经济损失。
