Sairam M R, Bhargavi G N
Science. 1985 Jul 5;229(4708):65-7. doi: 10.1126/science.2990039.
The biological properties of recombinants of glycoprotein hormones in which the alpha and beta subunits were differentially deglycosylated have been investigated. Specific deglycosylation of the alpha subunit generated a recombinant that had more receptor-binding activity but did not produce hormone response in the target cells. The deglycosylated alpha + beta recombinant was also an antagonist of the action of the native hormone. Thus, the carbohydrates in the alpha subunit play a dominant role in the transduction of the hormone signal into the cell.
对糖蛋白激素重组体的生物学特性进行了研究，其中α和β亚基进行了不同程度的去糖基化。α亚基的特异性去糖基化产生了一种重组体，其具有更高的受体结合活性，但在靶细胞中不产生激素反应。去糖基化的α + β重组体也是天然激素作用的拮抗剂。因此，α亚基中的碳水化合物在将激素信号转导到细胞中起主导作用。
