Department of Oncology, Shanxian Central Hospital, Heze, Shandong 274300, P.R. China.
Department of Medicine, Shanxian Central Hospital, Heze, Shandong 274300, P.R. China.
Mol Med Rep. 2018 Aug;18(2):2492-2498. doi: 10.3892/mmr.2018.9166. Epub 2018 Jun 14.
MicroRNA (miR)‑30a‑5p has been reported to suppress the progression of hepatocellular cancer, renal cell carcinoma, oral cancer and gastric cancer. However, whether miR‑30a‑5p is involved in the regulation of melanoma remains unclear. The present study revealed that miR‑30a‑5p was downregulated in melanoma tissues and cell lines. Overexpression of miR‑30a‑5p significantly inhibited the proliferation, migration and invasion of melanoma cells in vitro. In addition, ectopic expression of miR‑30a‑5p delayed tumor growth in vivo. In terms of mechanism, miR‑30a‑5p targeted sex determining region Y‑box 4 (SOX4) and impeded the expression of SOX4 in melanoma cells. In addition, SOX4 was upregulated in melanoma tissues and cell lines when compared with normal tissues or cells. Furthermore, overexpression of SOX4 significantly rescued the proliferation, migration and invasion of melanoma cells transfected with miR‑30a‑5p mimics. Taken together, the results of the present study demonstrated that miR‑30a‑5p suppressed the proliferation, migration and invasion of melanoma cells in SOX4‑dependent manner.
微小 RNA（miR）-30a-5p 已被报道可抑制肝细胞癌、肾细胞癌、口腔癌和胃癌的进展。然而，miR-30a-5p 是否参与调节黑色素瘤尚不清楚。本研究显示，miR-30a-5p 在黑色素瘤组织和细胞系中表达下调。miR-30a-5p 的过表达可显著抑制黑色素瘤细胞在体外的增殖、迁移和侵袭。此外，miR-30a-5p 的异位表达可延迟体内肿瘤的生长。就机制而言，miR-30a-5p 靶向性决定区 Y 框 4（SOX4）并抑制黑色素瘤细胞中 SOX4 的表达。此外，与正常组织或细胞相比，SOX4 在黑色素瘤组织和细胞系中上调。此外，过表达 SOX4 可显著挽救转染 miR-30a-5p 模拟物的黑色素瘤细胞的增殖、迁移和侵袭。综上所述，本研究结果表明，miR-30a-5p 通过 SOX4 依赖性途径抑制黑色素瘤细胞的增殖、迁移和侵袭。
