Pediatric Urology and Regenerative Medicine Research Center, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
J Biomed Mater Res A. 2018 Oct;106(10):2670-2678. doi: 10.1002/jbm.a.36464. Epub 2018 Sep 8.
Myocardial infarction (MI) is a major cause of mortality and morbidity in industrialized societies. Myocardial tissue engineering is an alternative and promising approach for substituting injured myocardium through development and seeding of appropriate scaffolds. In this study, we investigated the efficacy of using an acellular pericardium to deliver autologous mesenchymal stem cells (MSCs) to the infarcted site for regeneration of the myocardium. MI was induced in two groups of rats; G1 or MI group, and G2 or patch-implanted group. In G2 group, rats had undergone transplantation of a pericardial patch which was previously seeded with adipose tissue derived MSCs. To evaluate the efficacy of the pericardial patches, biopsies were taken one month after transplantation. In order to evaluate the extent of regeneration, inflammation and fibrosis, histopathological investigations including hematoxylin and eosin (H&E), Sirius Red and trichrome staining were performed. In addition, immunohistochemical investigations by Desmin as well as CD68, CD45 and CD34 antibodies were performed. Furthermore, Tunnel assay was performed to detect the extent of apoptosis. H&E assessments of biopsies from the patch-implanted group confirmed presence of pre-seeded pericardium containing MSCs along with neo-vessels. Immunohistochemical assessments demonstrated higher number of CD34 positive cells and Desmin-positive cells in the patch implanted group (p < 0.05); these findings are suggestive of cardiomyocyte regeneration in G2 rats. This study demonstrates the advantages of application of natural acellular scaffolds as cell delivery devices and it emphasizes neovascularization following this approach. However, further investigations are required to analyze long-term cardiac function in recipients. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2670-2678, 2018.
心肌梗死（MI）是工业化社会中死亡和发病的主要原因。心肌组织工程是一种替代方法，通过开发和播种适当的支架来替代受损的心肌，具有广阔的前景。在这项研究中，我们研究了使用去细胞心包作为载体将自体间充质干细胞（MSCs）递送到梗死部位以再生心肌的效果。我们在两组大鼠中诱导 MI；G1 或 MI 组，G2 或补片植入组。在 G2 组，大鼠接受了预先接种脂肪组织来源的 MSCs 的心包补片移植。为了评估心包补片的疗效，在移植后一个月进行了活检。为了评估再生、炎症和纤维化的程度，进行了苏木精和伊红（H&E）、天狼星红和三色染色的组织病理学研究。此外，还进行了 Desmin 以及 CD68、CD45 和 CD34 抗体的免疫组织化学研究。此外，还进行了Tunnel 检测以检测细胞凋亡的程度。补片植入组活检的 H&E 评估证实存在预先种植的含有 MSCs 的心包，以及新血管。免疫组织化学评估显示，补片植入组 CD34 阳性细胞和 Desmin 阳性细胞数量较多（p < 0.05）；这些发现提示 G2 大鼠的心肌细胞再生。这项研究证明了应用天然去细胞支架作为细胞输送装置的优势，并强调了这种方法后的新生血管化。然而，需要进一步的研究来分析受者的长期心脏功能。© 2018 Wiley Periodicals, Inc. J 生物材料 Res 部分 A：2670-2678，2018。
