Nguyen My Thi Ngoc, Doan Vu Nguyen, Tran Ha Le Bao
Department of Physiology and Animal Biotechnology, Faculty of Biology and Biotechnology, University of Science, Vietnam National University, Ho Chi Minh City Vietnam.
Laboratory of Tissue Engineering and Biomedical Materials, University of Science, Vietnam National University, Ho Chi Minh City Vietnam.
Turk J Biol. 2019 Dec 13;43(6):360-370. doi: 10.3906/biy-1908-10. eCollection 2019.
Bovine pericardium has been proposed as an available material for tissue engineering and bioprosthetic reconstruction. In this study, bovine pericardium was fabricated into a scaffold for culturing and chondrogenic differentiation of human adipose-derived stem cells (hADSCs). Bovine pericardium was treated in 10 mM Tris-HCl and 0.15% SDS, followed by crosslinking in 0.1% glutaraldehyde. Treated bovine pericardium (tBP) was characterized as a slight yellowish thin membrane with enhanced tensile strength and strain property. The membrane maintained stability under enzymatic conditions for up to 16 days of incubation. The results confirmed tBP as a cell-friendly scaffold for hADSCs due to low cytotoxicity and its ability to support an appropriate attachment and proliferation of hADSCs. Moreover, there was an accumulation of the extracellular matrix proteoglycan in tBP seeded with hADSCs after 7 and 14 days of chondrogenic induction. as a specific marker of chondrogenesis was detected after 7 days, whereas type X-a1 collagen () expression was stable up to day 14. However, minor expression of aggrecan was found. Taken together, these results indicate that tBP is a potential scaffold for hADSCs for cartilage tissue engineering.Key words: Bovine pericardium, scaffold, adipose-derived stem cells, chondrogenic differentiation, cartilage regeneration, augmentation rhinoplasty.
牛心包已被提议作为组织工程和生物假体重建的可用材料。在本研究中，牛心包被制成支架用于人脂肪来源干细胞（hADSCs）的培养和软骨分化。牛心包在10 mM Tris-HCl和0.15% SDS中处理，然后在0.1%戊二醛中交联。处理后的牛心包（tBP）被表征为略带黄色的薄膜，具有增强的拉伸强度和应变特性。该膜在酶促条件下孵育长达16天仍保持稳定性。结果证实tBP是一种对hADSCs细胞友好的支架，因为其细胞毒性低且能够支持hADSCs的适当附着和增殖。此外，在软骨诱导7天和14天后，接种hADSCs的tBP中细胞外基质蛋白聚糖有积累。在7天后检测到作为软骨形成特异性标志物，而X-a1型胶原（）的表达在第14天前保持稳定。然而，发现聚集蛋白聚糖的表达较少。综上所述，这些结果表明tBP是用于软骨组织工程的hADSCs的潜在支架。关键词：牛心包；支架；脂肪来源干细胞；软骨分化；软骨再生；隆鼻术。
