Busarakumtragul Panaree, Dusadeemethagul Jittra
J Med Assoc Thai. 2016 Nov;99 Suppl 8:S25-S30.
To evaluate the effects of the laughing training on serum cortisol and nitrite levels in Thai private office workers.
Forty volunteers age 25 to 60 years from private offices were recruited. Thirty eight remaining volunteers were divided into two groups: 20 for the experimental group and 18 for the control group. They were randomly assigned to participate in the training by matching up pairs of subjects with the same level of mean total scores from the Suanprung Stress Test-60 (SPT-60). The program ran and continued from June to July, 2013. The control subjects took a rest in the same room as the experimental group throughout the experiment. The experimental subjects participated in laughing training program for 3 days a week, 1 hour a day for 8 consecutive weeks. At 8.00 AM, eight milliliters of venous blood was drawn from individuals in both control and experimental groups at the beginning and at the end of the training program. Serum cortisol and nitrite were assayed by electro chemiluminescense immunoassay and enhance chemiluminescense using high sensitivity nitrite, respectively.
No significant difference was found in the mean of pre-post level of serum cortisol within control and experimental groups. Additionally, pre-level of serum nitrite in the experimental group revealed significant difference from post-level of serum nitrite in control and experimental groups at p<0.05.
Laughing training is supposed to promote good health by reducing acute or chronic stress during work. In this present study, laughing training for 8 weeks had significant effect on serum nitric oxide level indirectly determined by serum nitrite level. However, this laughing training program had no significant effect on the serum cortisol concentration.
评估大笑训练对泰国私营企业办公室职员血清皮质醇和亚硝酸盐水平的影响。
招募了40名年龄在25至60岁的私营企业办公室志愿者。其余38名志愿者被分为两组:实验组20人,对照组18人。通过将Suanprung压力测试-60(SPT-60)平均总分水平相同的受试者配对,随机分配他们参与训练。该项目于2013年6月至7月进行并持续。在整个实验过程中,对照组受试者与实验组在同一房间休息。实验组受试者每周参加3天大笑训练项目,每天1小时,连续8周。训练项目开始和结束时,上午8点从对照组和实验组的个体中抽取8毫升静脉血。血清皮质醇采用电化学发光免疫分析法测定,亚硝酸盐采用高灵敏度亚硝酸盐增强化学发光法测定。
对照组和实验组血清皮质醇前后水平均值无显著差异。此外,实验组血清亚硝酸盐的前水平与对照组和实验组血清亚硝酸盐的后水平相比,差异有统计学意义(p<0.05)。
大笑训练被认为可通过减轻工作期间的急性或慢性压力来促进身体健康。在本研究中,为期8周的大笑训练对通过血清亚硝酸盐水平间接测定的血清一氧化氮水平有显著影响。然而,该大笑训练项目对血清皮质醇浓度无显著影响。