人胃黏膜分离细胞产生H⁺的情况。

H+ production by isolated cells from human gastric mucosa.

作者信息

Schepp W, Miederer S E, Ruoff H J

出版信息

Agents Actions. 1985 Apr;16(3-4):191-4. doi: 10.1007/BF01983136.

DOI:10.1007/BF01983136

Abstract

Gastric mucosal cells were isolated from human mucosa obtained at surgery. H+ production was indirectly estimated by 14C aminopyrine (AP) uptake. The maximal response to histamine occurred after 30 min of incubation whereas intrinsic factor (IF) secretion was maximal after only 7.5 to 15 min. According to the concentration response curve 10(-4) mol/1 histamine proved to be the most effective concentration, the response to which was completely inhibited by ranitidine. Carbachol, dibutyryl cAMP and IMX also enhanced AP uptake, IMX being even more powerful than histamine. Carbachol and IMX failed to potentiate the response to histamine. Parietal cell fractions enriched by a Percoll density gradient revealed a pronounced background stimulation so that additional stimulation by test agents was less effective than in non-fractionized cells.

摘要

从手术获取的人体黏膜中分离出胃黏膜细胞。通过14C氨基比林（AP）摄取间接估算H+生成量。对组胺的最大反应在孵育30分钟后出现，而内因子（IF）分泌仅在7.5至15分钟后达到最大值。根据浓度反应曲线，10(-4) mol/1组胺被证明是最有效的浓度，雷尼替丁可完全抑制对其的反应。卡巴胆碱、二丁酰环磷腺苷（dibutyryl cAMP）和IMX也增强了AP摄取，IMX比组胺更有效。卡巴胆碱和IMX未能增强对组胺的反应。通过Percoll密度梯度富集的壁细胞组分显示出明显的背景刺激，因此测试剂的额外刺激在其中不如在未分级的细胞中有效。