The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Nucleic Acids Res. 2018 Sep 28;46(17):e103. doi: 10.1093/nar/gky513.
A polymerase ribozyme can be used to label the 3' end of RNA or DNA molecules by incorporating a variety of functionalized nucleotide analogs. Guided by a complementary template, the ribozyme adds a single nucleotide that may contain a fluorophore, biotin, azide or alkyne moiety, thus enabling the detection and/or capture of selectively labeled materials. Employing a variety of commercially available nucleotide analogs, efficient labeling was demonstrated for model RNAs and DNAs, human microRNAs and natural tRNA.
聚合酶核酶可通过掺入各种功能化核苷酸类似物来标记 RNA 或 DNA 分子的 3' 末端。在互补模板的指导下，核酶添加一个可能含有荧光团、生物素、叠氮化物或炔烃部分的单核苷酸，从而能够检测和/或捕获选择性标记的材料。利用各种市售的核苷酸类似物，对模型 RNA 和 DNA、人 microRNA 和天然 tRNA 进行了有效的标记。
