Mollova Emilia T
Department of Biochemistry, Stanford University, Stanford, CA 94305, USA.
Curr Opin Chem Biol. 2002 Dec;6(6):823-8. doi: 10.1016/s1367-5931(02)00394-0.
Less than a decade old, single-molecule fluorescence of nucleic acids has rapidly become an important tool in the arsenal of biological probes. A variety of novel approaches to investigate conformational dynamics, catalytic mechanisms, folding pathways and protein-nucleic-acid interactions have recently been devised for nucleic acids using this technique. Combined with biomechanical tools and ensemble measurements, single-molecule fluorescence methods extend our ability to observe and understand biomolecules and complex biological processes.
核酸的单分子荧光技术问世还不到十年，就迅速成为生物探针库中的一项重要工具。最近，利用这项技术设计出了各种新颖的方法来研究核酸的构象动力学、催化机制、折叠途径以及蛋白质 - 核酸相互作用。单分子荧光方法与生物力学工具和整体测量相结合，扩展了我们观察和理解生物分子及复杂生物过程的能力。
