Institute of Life Sciences and Resources & Department of Food Science and Biotechnology, College of Life Sciences , Kyung Hee University , Yongin 17104 South Korea.
J Agric Food Chem. 2018 Jul 5;66(26):6806-6813. doi: 10.1021/acs.jafc.8b01590. Epub 2018 Jun 25.
Herein, we report a fairly simple and environmentally friendly approach for the fabrication of starch-based magnetic polymer beads (SMPBs) with uniform shape and size through spontaneous rearrangement of short-chain glucan (SCG) produced by enzymatic debranching of waxy maize starch. The paramagnetic materials, dextran-coated iron oxide nanoparticles (Dex@IONPs), were readily incorporated into the starch microstructure and rendered a superparamagnetic property to the SMPBs. The morphology and size of resulting SMPBs turned out to be modulated by Dex@IONPs in a concentration-dependent manner, of which Dex@IONPs was assumed to be acting as a seed inducing the epitaxial crystallization of SCG and further transforming it into homogeneous microparticles. The surface of SMPBs was readily functionalized with an antibody through a one-step reaction using a linker protein. The immuno-SMPBs showed great capture efficiency (>90%) for target bacteria. The colloidal stability and favorable surface environment for biomolecules are believed to be responsible for the high capture efficiency and specificity of the SMPBs. Furthermore, the captured bacteria along with antibody and linker protein were effectively eluted from the surface of SMPBs by adding free maltose, making this new material suitable for various chromatographic applications.
本文报告了一种相当简单且环保的方法，通过酶解蜡质玉米淀粉产生的短链葡聚糖（SCG）的自发重排，制备具有均匀形状和尺寸的基于淀粉的磁性聚合物珠（SMPB）。超顺磁材料葡聚糖包覆的氧化铁纳米粒子（Dex@IONP）容易掺入淀粉微结构中，并赋予 SMPB 超顺磁性。结果表明，SMPB 的形态和尺寸可通过 Dex@IONP 浓度依赖性调节，Dex@IONP 被认为是作为诱导 SCG 外延结晶的种子，并进一步将其转化为均匀的微粒。SMPB 的表面可以通过使用连接蛋白的一步反应，很容易地与抗体进行功能化。免疫 SMPB 对目标细菌表现出很高的捕获效率（>90%）。胶体稳定性和有利于生物分子的表面环境被认为是 SMPB 具有高捕获效率和特异性的原因。此外，通过添加游离麦芽糖，可将抗体和连接蛋白从 SMPB 表面有效洗脱捕获的细菌，使这种新材料适用于各种色谱应用。
