Institute of Life Sciences and Resources & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 17104, South Korea.
Department of Food Science and Technology, Sejong University, Seoul, 05006, South Korea.
Colloids Surf B Biointerfaces. 2019 Apr 1;176:352-359. doi: 10.1016/j.colsurfb.2019.01.002. Epub 2019 Jan 8.
Starch microparticles (SMPs) of well-defined size and morphology were synthesized through pullulanase-mediated debranching of waxy maize starch followed by spontaneous re-assembly of the resulting short-chain glucan molecules in aqueous solution. Enzymatic debranching of amylopectins from native starch generated two major fractions corresponding to a smaller glucan and partially digested larger amylopectin molecules. The ratio of short-chain glucan (SCG) over partially digested amylopectin (PDAp) turned out to be the deterministic factors for the size and crystallinity of SMPs, of which the ratio could be controlled by the concentration of debranching enzyme. The PDAp fraction was closely associated with the creation of nuclei, determining the growth kinetics of SMPs which led to the formation of SMPs with a diameter ranging from 0.52.5 μm. In addition, we demonstrated that iron oxide nanoparticles (IONPs) were successfully incorporated into the starch microstructure by introducing them during the self-assembly reaction, conferring desired functionality onto the final SMPs. The incorporated IONPs rendered the SMPs an excellent magnetic sensitivity, which were successfully applied for the separation and concentration of target bacteria upon conjugation of specific antibody on the surface of SMPs. The simple processes and biocompatible nature of starch would make this approach attractive for many applications in the area of food, medicine and other related materials sciences.
通过支链淀粉酶介导的蜡质玉米淀粉解支，然后在水溶液中短链葡聚糖分子自发重新组装，合成了具有明确定义尺寸和形态的淀粉微球（SMPs）。从天然淀粉中支链淀粉酶解直链淀粉产生了两种主要的分数，对应于较小的葡聚糖和部分消化的较大直链淀粉分子。短链葡聚糖（SCG）与部分消化的直链淀粉（PDAp）的比例是 SMPs 尺寸和结晶度的决定性因素，通过分支酶的浓度可以控制该比例。PDAp 分数与核的形成密切相关，决定了 SMPs 的生长动力学，从而形成了直径为 0.52.5 μm 的 SMPs。此外，我们证明了通过在自组装反应过程中引入氧化铁纳米颗粒（IONPs），可以将 IONPs 成功地掺入淀粉微结构中，从而赋予最终 SMPs 所需的功能。掺入的 IONPs 使 SMPs 具有优异的磁敏性，成功地将其应用于表面结合特定抗体的 SMPs 对目标细菌的分离和浓缩。淀粉的简单工艺和生物相容性使其成为食品、医药和其他相关材料科学领域许多应用的有吸引力的方法。
