Graduate School of Biotechnology & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104 Republic of Korea.
Graduate School of Biotechnology & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104 Republic of Korea.
Carbohydr Polym. 2018 Feb 1;181:818-824. doi: 10.1016/j.carbpol.2017.11.073. Epub 2017 Nov 23.
Here, we report a simple and non-emulsion based approach to prepare starch-based magnetic polymer beads with well-defined size, shape and dispersibility in aqueous environment through co-precipitation of enzymatically synthesized short chain amylose (SCA) and dextran-coated iron oxide nanoparticles (Dex@IONPs). The size and morphology of magnetic polymer beads (MPBs) were controlled by employing Dex@IONPs as a seeding agent. The resulting superparamagnetic amylose microbeads (SAMBs) were readily functionalized with antibody through one step reaction using a linker protein, which showed great capture efficiency (>90%) and specificity for target bacteria present in complicated food matrix. The excellent magnetic sensitivity also enabled the SAMBs readily assembled into ordered 1D arrays by external magnetic field whose structure could permanently be fixed by SCA-mediated precipitation process.
在这里，我们报告了一种简单且无乳液的方法，通过酶合成的短链直链淀粉（SCA）和葡聚糖包覆的氧化铁纳米粒子（Dex@IONPs）共沉淀，在水相环境中制备具有良好尺寸、形状和分散性的淀粉基磁性聚合物珠。通过使用 Dex@IONPs 作为种子剂来控制磁性聚合物珠（MPBs）的尺寸和形态。所得超顺磁直链淀粉微珠（SAMBs）通过使用连接蛋白的一步反应很容易与抗体结合，对复杂食品基质中存在的目标细菌表现出高捕获效率（>90%）和特异性。优异的磁敏感性还使 SAMBs 能够通过外部磁场容易地组装成有序的一维阵列，其结构可以通过 SCA 介导的沉淀过程永久固定。
