Ramos Elizagaray Sabina I, Quiroga Patricia L, Pérez Roberto D, Sosa Carlos, Pérez Carlos A, Bongiovanni Guillermina A, Soria Elio A
a Universidad Nacional de Córdoba, Facultad de Ciencias Médicas , Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular , Córdoba , Argentina.
b Universidad Nacional de Córdoba , Facultad de Matemática, Astronomía y Física , Córdoba , Argentina.
J Diet Suppl. 2019;16(4):401-407. doi: 10.1080/19390211.2018.1470124. Epub 2018 Jun 29.
Arsenic (As) is a worldwide immunotoxic agent that is in contaminated waters and consumed by mammals. Phytotherapy may counteract its harmful effects. Stuck (LG, ) and its extract are proposed as protective, given vvits bioactivity. The aim was to determine the protective capacity of the aqueous LG extract on splenocytes exposed to arsenic. Splenocytes were obtained from an arsenicosis model (Wistar rats consuming orally 0 [control; C] or 5 mg/Kg/d of As) that received 0-100 mg/Kg/d of LG extract for 30 days. As content (total reflection X-ray fluorescence), fatty acid profile (gas chromatography), γ-glutamyl transpeptidase activity (Szasz method), peroxides (xylenol orange-based assay), and nitrites (Griess reaction) were then assayed in viable splenocytes. Data were analyzed with ANOVA and the Tukey's test ( < .05). It was observed that the splenocytes contained 2.2 mg/Kg of this elemental arsenic. With γ-glutamyl transpeptidase inhibition and consequent triggering of hydroperoxides ( < .05), it was observed to increase saturated fatty acids and alter lipid profiles. LG treatment avoided damaging effects with values similar to unexposed C ( < .05), and cellular arsenic concentration ( < .0001). In conclusion, the aqueous extract of counteracted arsenic toxicity in rat splenocytes by preventing its cellular accumulation and induction of lipid and redox disturbances, which may impair immune function.
