DeMartinis F D
Am J Physiol. 1985 Jul;249(1 Pt 1):C89-96. doi: 10.1152/ajpcell.1985.249.1.C89.
To facilitate investigations on very small fat cell (VSFC) populations in adipose tissue, an alternate method of preparing fat tissue samples was explored. The osmium tetroxide-8M urea method, modified by addition of a 95% ethanol step in tissue processing, centrifugation between steps, and final resuspension in 55% glycerol in 0.01% Triton-saline, was compared with the collagenase method for determination of VSFC populations in Fischer 344 epididymal and Sprague-Dawley retroperitoneal adipose depots. For each method and in both depots, the average histogram of 300 adipocyte diameters, measured by microscopy, was bimodal with the nadir between 30 and 40 micron diameter. The average histogram of fat cells less than 35 micron in diameter showed a separate population of VSFC existed in each depot. The modified osmium-urea method gave better results and was easier to perform than the collagenase method. It has confirmed our earlier results and raises anew questions concerning a role for the natural existence of a VSFC population in the adipose depot.
为便于对脂肪组织中极小脂肪细胞（VSFC）群体进行研究，探索了一种制备脂肪组织样本的替代方法。将四氧化锇-8M尿素法进行了改进，即在组织处理过程中增加一个95%乙醇步骤、在步骤之间进行离心，并最终重悬于含0.01% Triton的55%甘油盐溶液中，将其与胶原酶法进行比较，以测定Fischer 344大鼠附睾脂肪库和Sprague-Dawley大鼠腹膜后脂肪库中的VSFC群体。对于每种方法以及在两个脂肪库中，通过显微镜测量的300个脂肪细胞直径的平均直方图均为双峰，最低点位于直径30至40微米之间。直径小于35微米的脂肪细胞平均直方图显示，每个脂肪库中都存在一个独立的VSFC群体。改进后的锇-尿素法比胶原酶法效果更好且操作更简便。它证实了我们早期的结果，并再次引发了关于脂肪库中天然存在VSFC群体的作用的问题。
