Krakower G R, James R G, Arnaud C, Etienne J, Keller R H, Kissebah A H
Department of Medicine, Medical College of Wisconsin, Milwaukee 53226.
J Clin Invest. 1988 Mar;81(3):641-8. doi: 10.1172/JCI113367.
A flow cytometric immunofluorescence procedure utilizing a specific antibody to rat adipose tissue lipoprotein lipase (LPL) was developed to quantify differentiated and undifferentiated preadipocytes present in the adipose tissue vascular stroma. This method is highly sensitive and specific for cells capable of synthesizing LPL in significant quantities. Pubescence in female rats was associated with an increase in differentiated preadipocytes and in fat cell number with enlargement of the fat depots in the perirenal, parametrial, and the subcutaneous dorsal and femoral regions. A concomitant decline in the percentage of undifferentiated preadipocytes occurred in all but the femoral depot. Ovariectomy reduced pubertal adipose growth in the femoral and parametrial but not the dorsal or perirenal regions. Furthermore, the femoral undifferentiated preadipocyte pool was not preserved in the ovariectomized animals. Thus, ovarian factors influence the pubescence-associated regional preadipocyte differentiation and conversion to adipocytes. The femoral depot contains an ovarian-dependent infinite pool of fat cell precursors. These features could account for the association between ovarian hormones and body fat topography.
利用抗大鼠脂肪组织脂蛋白脂肪酶（LPL）的特异性抗体开发了一种流式细胞免疫荧光程序，以定量脂肪组织血管基质中存在的分化和未分化前脂肪细胞。该方法对能够大量合成LPL的细胞具有高度敏感性和特异性。雌性大鼠的青春期与分化的前脂肪细胞增加、脂肪细胞数量增加以及肾周、子宫旁、皮下背部和股骨区域的脂肪库增大有关。除股骨脂肪库外，所有其他部位未分化前脂肪细胞的百分比均随之下降。卵巢切除术减少了股骨和子宫旁青春期的脂肪生长，但对背部或肾周区域没有影响。此外，在去卵巢动物中，股骨未分化前脂肪细胞库未得到保留。因此，卵巢因素影响青春期相关的区域前脂肪细胞分化和向脂肪细胞的转化。股骨脂肪库包含一个依赖卵巢的无限脂肪细胞前体池。这些特征可以解释卵巢激素与身体脂肪分布之间的关联。
