Hirsch J, Gallian E
J Lipid Res. 1968 Jan;9(1):110-9.
Four methods for the sizing of adipose cells in small samples of human or animal adipose tissue are compared. These methods depend on the preparation of cell suspensions by incubation of the tissue with collagenase or by prolonged fixation with osmium tetroxide and separation of the fixed cells. A Coulter electronic counter was used to count and size the suspended cells and a Zeiss particle size analyzer for the sizing of cells in photomicrographs. The use of the Coulter counter to count cells in a suspension derived from a known amount of tissue and subjected to osmium tetroxide fixation is recommended for accuracy and general applicability to adipose cells of all sizes in man and animals.
比较了四种用于测定人或动物小样本脂肪组织中脂肪细胞大小的方法。这些方法依赖于通过用胶原酶孵育组织或用四氧化锇长时间固定并分离固定细胞来制备细胞悬液。使用库尔特电子计数器对悬浮细胞进行计数和测定大小，并使用蔡司粒度分析仪对显微照片中的细胞进行大小测定。建议使用库尔特计数器对来自已知量组织并经过四氧化锇固定的悬液中的细胞进行计数，因为其准确性高且普遍适用于人和动物各种大小的脂肪细胞。
