USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32 El-Alia, Bab Ezzouar, Algiers, Algeria.
USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32 El-Alia, Bab Ezzouar, Algiers, Algeria.
Int Immunopharmacol. 2018 Aug;61:256-265. doi: 10.1016/j.intimp.2018.06.013. Epub 2018 Jun 11.
Antivenom treatment has been largely used against scorpion stings. Despite their efficacy, the use of mammalian antivenoms may cause adverse effects due to the immune system activation. IgYs from hyperimmunized laying hens against venoms could be a promising alternative to equine IgGs due to the various benefits that these antibodies can provide. Here we report the preparation of specific IgYs by immunizing laying hens with Aah (Androctonus australis hector) scorpion venom. IgYs were isolated from egg yolks by water dilution and salt precipitation methods; they were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis, western blot and ELISA. The efficiency of these immunoglobulins on the pathophysiological effects induced by Aah venom was assessed by histological and metabolical analysis of the aorta and the heart. The inflammatory response was assessed by evaluating the granulocyte tissue infiltration and oxidative/nitrosative status. Results revealed high IgYs titers against Aah venom by ELISA. Overall, these IgYs seem to protect efficiently mice against envenomation and neutralized the lethal effects of scorpion venom with a high efficacy; the median effective dose (ED50) was 221 μl/2 LD50; i.e. an amount of 79.23 mg of IgY scan neutralize 1 mg of Aah venom. IgY antibodies neutralize effectively the Aah venom lethality and could prevent severe pathological effects induced by scorpion venom and could be used as an effective alternative to equine IgGs against scorpion envenoming.
抗蛇毒血清治疗在很大程度上被用于对抗蝎子蜇伤。尽管它们具有疗效，但由于免疫系统的激活，使用哺乳动物抗蛇毒血清可能会引起不良反应。针对毒液的免疫母鸡产生的 IgY 可以替代马 IgGs，因为这些抗体可以提供多种益处。在这里，我们报告了通过用 Aah（Androctonus australis hector）蝎子毒液免疫母鸡来制备特异性 IgY 的方法。通过水稀释和盐沉淀法从蛋黄中分离出 IgY；通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳、western blot 和 ELISA 对其进行了表征。通过对主动脉和心脏的组织学和代谢分析评估这些免疫球蛋白对 Aah 毒液引起的病理生理效应的效率。通过评估粒细胞组织浸润和氧化/硝化状态来评估炎症反应。ELISA 结果显示针对 Aah 毒液的 IgY 效价很高。总的来说，这些 IgY 似乎能有效地保护小鼠免受毒液侵害，并以高疗效中和了蝎子毒液的致死作用；半数有效剂量（ED50）为 221μl/2LD50；即 79.23mg 的 IgY 可以中和 1mg 的 Aah 毒液。IgY 抗体能有效中和 Aah 毒液的致死作用，并能预防蝎子毒液引起的严重病理作用，可作为对抗蝎子蜇伤的马 IgGs 的有效替代品。
