Yan Jiuming, Yue Qianlan, Jia Zhenchao, Chen Jinyao, Zhang Lishi
West China School of Public Health, Sichuan University, Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu 610041, China.
Wei Sheng Yan Jiu. 2016 Jul;45(4):631-636.
To evaluate of bamboo charcoal powder( BCP) on the lipid profile and mechanism in hyperlipidaemia model rats.
40 male Sprague-Dawley( SD) animals of 4 weeks old were randomly assigned into five groups: the control group fed with low-fat diet; the model control group and the test group( 2. 81, 5. 62, 11. 24 g /kg). Each group gived BCP or distilled water correspondingly, the total administration duration was 90 consecutive days. After the blood samples were collected, liver, kidneys, and white adipose around bilateral epididymis and kidneys were excised and weighed. Serum biomarkers of liver and kidney function were detected. The activities of TC, TGand MDA, T-AOC, CAT, SOD of liver were determined by corresponding test kits according to the manufacturer's protocols. Livers were also further detected by macroscopic and microscopic examinations.
After 90 days of treatment, the weight of rats more than 4 weeks, liver weight and percentage of body fat, serum AST, TG and VLDL, hepatic MDA, TG, TC and liver steatosis in the model control group was all increased compared with the negative control group, indicating that the model has been successfully built. It showed that the weight of rats, liver weight and white adipose weight, serum AST, TG and VLDL, hepatic MDA, TC and liver steatosis in the three dose group was all decreased. The hepatic SOD, CAT, T-AOC in the three dose groups were all increased.
The BCP could reduce the accumulation of body fat, inmprove blood lipid and hepatic steatosis in hyperlipidemia model rats.
评估竹炭粉（BCP）对高脂血症模型大鼠血脂水平及作用机制的影响。
将40只4周龄雄性Sprague-Dawley（SD）大鼠随机分为五组：对照组给予低脂饮食；模型对照组和试验组（2.81、5.62、11.24 g/kg）。每组分别给予相应的竹炭粉或蒸馏水，连续给药90天。采集血样后，切除肝脏、肾脏以及双侧附睾和肾脏周围的白色脂肪并称重。检测肝肾功能的血清生物标志物。按照试剂盒说明书，使用相应试剂盒测定肝脏中TC、TG和MDA、T-AOC、CAT、SOD的活性。还对肝脏进行大体和显微镜检查。
治疗90天后，与阴性对照组相比，模型对照组4周以上大鼠体重、肝脏重量和体脂百分比、血清AST、TG和VLDL、肝脏MDA、TG、TC及肝脏脂肪变性均增加，表明模型成功建立。结果显示，三个剂量组大鼠体重、肝脏重量和白色脂肪重量、血清AST、TG和VLDL、肝脏MDA、TC及肝脏脂肪变性均降低。三个剂量组肝脏SOD、CAT、T-AOC均升高。
竹炭粉可减少高脂血症模型大鼠体内脂肪堆积，改善血脂及肝脏脂肪变性。
