Department of Gastroenterology, Health Research Institute of the Hospital Clínico San Carlos (IdISSC), Hospital Clínico San Carlos, Madrid, Spain.
Laboratory of research in Complex Disease Genetics, Health Research Institute of the Hospital Clínico San Carlos (IdISSC), Hospital Clínico San Carlos, Madrid, Spain.
Dig Liver Dis. 2018 Nov;50(11):1183-1188. doi: 10.1016/j.dld.2018.04.014. Epub 2018 Apr 27.
To diagnose coeliac disease (CD) in individuals on a gluten free diet (GFD), we aimed to assess the utility of detecting activated γδ and CD8 T cells expressing gut-homing receptors after a short gluten challenge.
We studied 15 CD patients and 35 non-CD controls, all exposed to three days of gluten when following a GFD. Peripheral blood was collected before and six days after starting gluten consumption, and the expression of CD103, β7 and CD38 in γδ and CD8 T cells was assessed by flow cytometry. Determination of IFN-γ and IP-10 was performed by means of ELISPOT and/or Luminex technology.
We observed both γδ and CD8 T cells coexpressing CD103, β7 and CD38 in every patient with CD on day six, but only in one control. The studied CD8 T subpopulation was easier to detect than the γδ subpopulation. Increased IFN-γ and IP-10 levels after challenge were observed in patients with CD, but not in controls.
A short three-day gluten challenge elicits the activation of CD103 β7 CD8 T cells in CD. These cells can be detected by flow cytometry in peripheral blood, opening new possibilities for CD diagnosis in individuals on a GFD.
为了在接受无麸质饮食（GFD）的个体中诊断乳糜泻（CD），我们旨在评估在短期麸质挑战后检测表达肠道归巢受体的活化 γδ 和 CD8 T 细胞的效用。
我们研究了 15 名 CD 患者和 35 名非 CD 对照者，他们在接受 GFD 时均接触了三天的麸质。在开始摄入麸质前和摄入麸质后 6 天采集外周血，并通过流式细胞术评估 γδ 和 CD8 T 细胞中 CD103、β7 和 CD38 的表达。通过 ELISPOT 和/或 Luminex 技术测定 IFN-γ 和 IP-10。
我们观察到，在第 6 天，每个 CD 患者的 γδ 和 CD8 T 细胞均共表达 CD103、β7 和 CD38，但仅在一个对照者中观察到。研究的 CD8 T 亚群比 γδ 亚群更容易检测到。在 CD 患者中观察到挑战后 IFN-γ 和 IP-10 水平升高，但在对照者中没有。
为期三天的短期麸质挑战会引发 CD 患者中 CD103 β7 CD8 T 细胞的活化。这些细胞可以在外周血中通过流式细胞术检测到，为 GFD 个体中的 CD 诊断开辟了新的可能性。
