Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.
Am J Reprod Immunol. 2018 Oct;80(4):e12998. doi: 10.1111/aji.12998. Epub 2018 Jun 14.
The role of ILT4 DCs in healthy fertile controls and patients with recurrent miscarriages (RM) and recurrent implantation failure (RIF) is unclear.
We studied the expression of ILT4 from peripheral blood and endometrial samples from healthy controls and patients with RM and RIF by flow cytometry and immunohistochemistry analysis. Endometrial Foxp3 expression was also investigated using immunohistochemistry.
In peripheral blood, there was a significant increase in the percentage of ILT4 DCs in healthy fertile controls compared with patients with RM and RIF. The presence of ILT4 DC is even more prominent in the endometrium of healthy fertile controls compared with patients with RM and RIF. Moreover, there was a strong correlation between the number of ILT4 cells and Foxp3 Tregs in healthy fertile controls, but not in patients with RM and RIF.
Our data indicate that ILT4 DCs play an important role in the maintenance of immune tolerance during pregnancy, probably through the induction of Foxp3 Treg cells, a process which is impaired in RM and RIF.
ILT4 DCs 在健康生育控制者以及复发性流产（RM）和反复着床失败（RIF）患者中的作用尚不清楚。
我们通过流式细胞术和免疫组化分析研究了健康对照组和 RM 和 RIF 患者外周血和子宫内膜样本中 ILT4 的表达。还使用免疫组化研究了子宫内膜 Foxp3 的表达。
在外周血中，与 RM 和 RIF 患者相比，健康生育控制者中 ILT4 DC 的比例显著增加。与 RM 和 RIF 患者相比，ILT4 DC 在健康生育控制者的子宫内膜中更为突出。此外，在健康生育控制者中，ILT4 细胞的数量与 Foxp3 Treg 之间存在很强的相关性，但在 RM 和 RIF 患者中则没有。
我们的数据表明，ILT4 DCs 在怀孕期间维持免疫耐受中发挥重要作用，可能通过诱导 Foxp3 Treg 细胞，这一过程在 RM 和 RIF 中受损。
