Vidal M, Bienvenue A, Sainte-Marie J, Philippot J
Eur J Biochem. 1984 Jan 16;138(2):399-405. doi: 10.1111/j.1432-1033.1984.tb07929.x.
The release of the internal content of negatively charged phosphatidylcholine/phosphatidylserine vesicles under the influence of high density lipoprotein was studied. Under standard conditions (the same composition outside and inside the compartment) the leakage of negative liposomes increased significantly. However, a high internal concentration of calcein provoked a sealing effect, exhibited both in sucrose and in calcein release. This sealing effect is not related to the size of vesicles, the fluidity of the membrane, the distribution of phosphatidylserine molecules, or the membrane potential. Our data indicate that surface potential influences this effect, probably in addition to a lateral pressure effect such as with cholesterol. The surface potential, as measured by the water-lipid partition coefficient of fatty acids, is strongly affected by internal ionic strength when liposomes contain calcein as well as other polyanions (6-carboxyfluorescein, sodium citrate).
研究了在高密度脂蛋白影响下带负电荷的磷脂酰胆碱/磷脂酰丝氨酸囊泡内部内容物的释放情况。在标准条件下（隔室内外组成相同），负性脂质体的泄漏显著增加。然而，高浓度的内部钙黄绿素引发了一种封闭效应，在蔗糖和钙黄绿素释放中均有体现。这种封闭效应与囊泡大小、膜流动性、磷脂酰丝氨酸分子分布或膜电位无关。我们的数据表明，表面电位可能除了像胆固醇那样的侧向压力效应外，还会影响这种效应。当脂质体含有钙黄绿素以及其他聚阴离子（6 - 羧基荧光素、柠檬酸钠）时，通过脂肪酸的水 - 脂质分配系数测量的表面电位会受到内部离子强度的强烈影响。
