[铅中毒对幼鼠脑线粒体特性的影响]

[Effects of lead poisoning on properties of brain mitochondria in young rats].

作者信息

Dumas P, Gueldry D, Loireau A, Chomard P, Buthieau A M, Autissier N

出版信息

C R Seances Soc Biol Fil. 1985;179(2):175-83.

Abstract

When the litters were 14 days old, wistar rat pups received lead ions. The suckling mother was fed by a powdered laboratory chow containing lead carbonate (1%, 2%, 4%). The intoxication was going on for 3 days to 9 weeks. While lead-fed pups showed a decrease in body-weight gain and in neurologic development, lead ions were without effects on oxygen consumption, ADP-phosphorylation and activity of several enzymes of cerebral mitochondria. On the other hand, the important lead accumulation into cerebral mitochondria was in a straight ratio with lead doses in the feedings. To explain the in vivo data, the effects of lead acetate were investigated in vitro on brain mitochondria of young male wistar rats. Our findings indicate that lead ions alter mitochondrial respiration and ADP-phosphorylation and that inorganic phosphate has a protective effect.

摘要

当幼崽14天大时，Wistar大鼠幼崽接受铅离子。哺乳的母鼠喂食含有碳酸铅（1%、2%、4%）的实验室粉状饲料。中毒持续3天至9周。虽然喂食铅的幼崽体重增加和神经发育有所下降，但铅离子对氧消耗、ADP磷酸化和脑线粒体几种酶的活性没有影响。另一方面，脑线粒体中铅的重要积累与饲料中的铅剂量成正比。为了解释体内数据，在体外研究了醋酸铅对年轻雄性Wistar大鼠脑线粒体的影响。我们的研究结果表明，铅离子会改变线粒体呼吸和ADP磷酸化，无机磷酸盐具有保护作用。