Preclinical and Translational Pharmacology, Department of Pharmacy, Health Science and Nutrition, University of Calabria, 87036 Rende, CS, Italy.
Department of Physiology and Anatomy, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan.
Fitoterapia. 2018 Sep;129:20-24. doi: 10.1016/j.fitote.2018.06.007. Epub 2018 Jun 12.
Bergamot essential oil (BEO) has proven wide evidence of pharmacological antinociceptive effectiveness both in nociceptive and in neuropathic pain models. The antinociceptive properties of BEO for inhalation have not been investigated. The purpose of this study is to evaluate the effects of the inhalation of BEO on formalin-induced nociceptive response in mice. Male ddY-strain mice (Japan SLC, Hamamatsu, Japan) of 23-25 g of weight at the time the experiments underwent the formalin test. Twenty μl of formalin (2% in saline) were administered into the plantar surface of the mice hindpaw and the time of licking/biting was observed and recorded at intervals of 5 min. The device for BEO inhalatory delivery consisted in a filter paper disc soaked with known volume of BEO placed on the edge of the cage. Inhalation of BEO exerted antinociceptive activity. In particular, it reduced the formalin-induced licking/biting behaviour in a manner that was dependent on the volume of BEO used in the device for its release and on the time of exposure to the phytocomplex. The results support the use of BEO in aromatherapy for complementary management of chronic pain relief in a stepwise therapeutic programme.
佛手柑精油（BEO）在伤害感受和神经病理性疼痛模型中均已证明具有广泛的药理学镇痛作用。BEO 吸入的镇痛特性尚未被研究。本研究旨在评估吸入 BEO 对小鼠福尔马林诱导的疼痛反应的影响。雄性 ddY 品系小鼠（日本 SLC，滨松，日本）在进行福尔马林试验时体重为 23-25g。将 20μl 福尔马林（2%在生理盐水中）注入小鼠后爪足底表面，每隔 5 分钟观察并记录舔/咬时间。BEO 吸入给药装置由浸泡在已知体积 BEO 中的滤纸盘组成，放置在笼子边缘。吸入 BEO 表现出镇痛活性。特别是，它以依赖于用于释放植物复合物的装置中 BEO 体积和暴露于植物复合物的时间的方式减少了福尔马林诱导的舔/咬行为。这些结果支持在芳香疗法中使用 BEO，以在逐步治疗方案中补充管理慢性疼痛缓解。
