Department of Chemical Science and Technologies, University of Rome "Tor Vergata", Via della Ricerca Scientifica 1, 00133, Rome, Italy.
Department of Chemical Science and Technologies, University of Rome "Tor Vergata", Via della Ricerca Scientifica 1, 00133, Rome, Italy.
Anal Chim Acta. 2018 Oct 31;1029:1-7. doi: 10.1016/j.aca.2018.04.065. Epub 2018 May 4.
The development of portable and user-friendly sensing platforms is a hot topic in the field of analytical chemistry. Among others, electroanalytical approaches exhibit a high amenability for reaching this purpose, i.e. the commercial strips for diabetes care are an obvious success. However, providing fully-integrated and user-friendly methods is the leitmotiv. In this work we evaluate the use of a disposable pipette tip, opportunely configured, to realize the first example of lab-on-a-tip. The combination of a pipette tip, wire electrodes, and cotton wool filter, highlights the suitability of producing a novel one-shot electroanalytical platform that does not require expertise-required tasks. To demonstrate the feasibility of this novel method, copper (Cu) is detected in water samples by means of anodic stripping voltammetry. The quantification is performed directly into the tip that contains a cotton wool filter: the filter has the double function of purifying the matrices from gross impurities and releasing all the pre-loaded reagents necessary for the assay. After optimizing the experimental parameters, the lab-on-a-tip was capable of detecting Cu linearly up to 300 μg/L with a detection limit of 6.3 μg/L. The effectiveness of the platform was confirmed by testing 50, 100, and 150 ppb Cu-spiked river water sample with recovery value comprised between 92 and 103%.
便携式和用户友好型传感平台的发展是分析化学领域的热门话题。在其他方法中，电化学生成法非常适合实现这一目标，例如用于糖尿病护理的商业检测条就是一个明显的成功案例。然而，提供完全集成和用户友好的方法是关键。在这项工作中，我们评估了使用一次性移液器吸头（经过适当配置）来实现首个片上实验室的示例。移液器吸头、金属丝电极和棉花过滤材料的组合，突出了生产新型一次性电分析平台的适用性，这种平台不需要专业技能。为了证明这种新型方法的可行性，我们通过阳极溶出伏安法在水样中检测铜（Cu）。通过将包含棉花过滤材料的吸头直接进行定量分析，可以实现定量分析：过滤材料具有双重功能，既能从基质中去除大的杂质，又能释放出测定所需的所有预加载试剂。在优化实验参数后，该片上实验室能够在线性范围内检测到 300μg/L 的 Cu，检测限为 6.3μg/L。通过测试 50、100 和 150 ppb Cu 加标河水样品，并得到 92%至 103%的回收率值，验证了该平台的有效性。
