Yield10 Bioscience, Inc., 19 Presidential Way, Woburn, MA 01801, United States.
Yield10 Bioscience, Inc., 19 Presidential Way, Woburn, MA 01801, United States.
Plant Sci. 2018 Aug;273:100-109. doi: 10.1016/j.plantsci.2018.04.003. Epub 2018 Apr 7.
Increasing crop yield requires the coordination of multiple metabolic pathways spanning photosynthetic carbon fixation, central carbon metabolism, and finally targeted carbon deposition to end product. In this study, we used a transcriptome-based gene regulatory association network to search for transcription factor genes that could play a role in increasing carbon flow through pathways associated with these processes to increase biomass yield in switchgrass. Two novel switchgrass transcription factors, PvBMY1 (BioMass Yield 1, belonging to the APETALA2/Ethylene Response Factor family of transcription factors) and PvBMY3 (BioMass Yield 3, a member of the Nuclear-Factor Y family of transcription factors), with predicted roles in the regulation of photosynthesis and related metabolism were identified. These genes were overexpressed in switchgrass to determine their impact on biomass yield. A significant increase in both aboveground and root biomass was observed in transgenic greenhouse grown plants compared to wild-type control plants with the best line producing 160% more aboveground biomass than controls. Transgenic lines with elevated electron transport rate of photosystems I and II as well as increased levels of starch and soluble sugars were identified.
提高作物产量需要协调多个代谢途径，包括光合作用碳固定、中心碳代谢，最终将碳定向沉积到终产物。在这项研究中，我们使用基于转录组的基因调控关联网络来搜索转录因子基因，这些基因可能在增加与这些过程相关的途径中的碳流方面发挥作用，从而提高柳枝稷的生物量产量。鉴定出两个新型柳枝稷转录因子 PvBMY1（生物量产量 1，属于 APETALA2/乙烯响应因子家族的转录因子）和 PvBMY3（生物量产量 3，核因子 Y 家族的转录因子成员），它们在光合作用和相关代谢的调节中具有预测作用。这些基因在柳枝稷中过表达，以确定它们对生物量产量的影响。与野生型对照植物相比，温室生长的转基因植物的地上部和根生物量均显著增加，最好的品系比对照植物多产生 160%的地上部生物量。鉴定出具有较高的光系统 I 和 II 电子传递速率、较高的淀粉和可溶性糖水平的转基因株系。
