College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
Horticulture Section, School of Integrative Plant Science, Cornell University, Ithaca, USA.
Sci Rep. 2018 Jun 15;8(1):9187. doi: 10.1038/s41598-018-27530-x.
Flooding severely limits plant growth even for some aquatic plants. Although much work has been done on submergence response of some important crop plants, little is known about the response mechanism of aquatic plants, i.e. lotus (Nelumbo nucifera). In this study, we investigated the genome-wide regulation lotus genes in response to submergence stress by high-throughput mRNA sequencing. A total of 4002 differentially expressed genes (DEGs) in lotus upon submergence stress. Among them, 1976 genes were up-regulated and 2026 down-regulated. Functional annotation of these genes by Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis revealed that they were mainly involved in processes of oxidation-reduction, abiotic stimuli, cellular metabolism and small molecule metabolism. Based on these data, previous work and quantitative RT-PCR (RT-qPCR) validation, we constructed a cooperative regulation network involved in several important DEGs in regards to the antioxidant system, disease resistance, hypoxia resistance and morphological adaptation. Further work confirmed that several innate immunity genes were induced during submergence and might confer higher resistance to lotus rot disease. In conclusion, these results provide useful information on molecular mechanisms underlying lotus responses to submergence stress.
洪水严重限制了植物的生长，即使对于一些水生植物也是如此。尽管已经对一些重要作物的淹没反应进行了大量研究，但对水生植物（如荷花）的反应机制却知之甚少。在这项研究中，我们通过高通量 mRNA 测序研究了荷花在淹没胁迫下的全基因组调控基因。在淹没胁迫下，荷花中共有 4002 个差异表达基因（DEGs）。其中，1976 个基因上调，2026 个基因下调。通过基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析对这些基因进行功能注释，表明它们主要参与氧化还原、非生物刺激、细胞代谢和小分子代谢过程。基于这些数据、以前的工作和定量 RT-PCR（RT-qPCR）验证，我们构建了一个涉及几个重要 DEGs 的合作调控网络，这些基因与抗氧化系统、抗病性、耐缺氧性和形态适应有关。进一步的工作证实，在淹没过程中诱导了几个先天免疫基因，这可能赋予荷花更高的腐烂病抗性。总之，这些结果为荷花对淹没胁迫反应的分子机制提供了有用的信息。
