Jin Qijiang, Xu Yingchun, Mattson Neil, Li Xin, Wang Bei, Zhang Xiao, Jiang Hongwei, Liu Xiaojing, Wang Yanjie, Yao Dongrui
College of Horticulture, Nanjing Agricultural University Nanjing, China.
Horticulture Section, School of Integrative Plant Science, Cornell University New York, NY, USA.
Front Plant Sci. 2017 Jan 18;8:6. doi: 10.3389/fpls.2017.00006. eCollection 2017.
MicroRNAs (miRNAs) are endogenous non-coding RNAs with important regulatory functions in plant development and stress responses. However, their population abundance in lotus ( Gaertn) has so far been poorly described, particularly in response to stresses. In this work, submergence-related miRNAs and their target genes were systematically identified, compared, and validated at the transcriptome-wide level using high-throughput sequencing data of small RNA, Mrna, and the degradome. A total of 128 known and 20 novel miRNAs were differentially expressed upon submergence. We identified 629 target transcripts for these submergence-responsive miRNAs. Based on the miRNA expression profiles and GO and KEGG annotation of miRNA target genes, we suggest possible molecular responses and physiological changes of lotus in response to submergence. Several metabolic, physiological and morphological adaptations-related miRNAs, i.e., NNU_far-miR159, NNU_gma-miR393h, and NNU_aly-miR319c-3p, were found to play important regulatory roles in lotus response to submergence. This work will contribute to a better understanding of miRNA-regulated adaption responses of lotus to submergence stress.
微小RNA（miRNA）是一类内源性非编码RNA，在植物发育和应激反应中具有重要的调控功能。然而，迄今为止，它们在莲（Nelumbo nucifera Gaertn）中的群体丰度描述甚少，尤其是在应对胁迫方面。在这项研究中，利用小RNA、mRNA和降解组的高通量测序数据，在全转录组水平上系统地鉴定、比较和验证了与淹水相关的miRNA及其靶基因。共有128个已知miRNA和20个新miRNA在淹水时差异表达。我们鉴定出了这些淹水响应性miRNA的629个靶转录本。基于miRNA表达谱以及miRNA靶基因的GO和KEGG注释，我们提出了莲在淹水时可能的分子响应和生理变化。发现了几种与代谢、生理和形态适应相关的miRNA，即NNU_far-miR159、NNU_gma-miR393h和NNU_aly-miR319c-3p，它们在莲对淹水的响应中发挥重要调控作用。这项工作将有助于更好地理解miRNA调控的莲对淹水胁迫的适应反应。
