Liu Ying, Cai Wei, Li Ning, Liu Si-yi, Wang Zi-jian, Liu Zhen-qing, Zhang Jia-yu, Zhao Bao-sheng
Yao Xue Xue Bao. 2016 Nov;51(11):1751-8.
The method of UHPLC-LTQ-Orbitrap mass spectrometry coupled with higher energy collision dissociation (HCD) was established to rapidly analyze the constituents absorbed into blood after oral administration of steroidal saponins from Radix Ophiopogonis. A total of 31 constituents, including 13 furostanol steroidal saponins and 18 spirostanol steroidal saponins, were characterized based on the accurate mass measurements, fragmentation patterns, chromatographic retention times, and diagnostic product ions. Among them, 8 compounds were unambiguously identified by comparison with their corresponding standards. The results provide comprehensive insights and guidance for elucidation of material basis of Radix Ophiopogonis activity.
建立了超高效液相色谱-线性离子阱-轨道阱质谱联用高能碰撞解离（HCD）方法，用于快速分析麦冬甾体皂苷口服给药后吸收入血的成分。基于精确质量测量、碎片模式、色谱保留时间和诊断产物离子，共鉴定出31种成分，包括13种呋甾烷醇甾体皂苷和18种螺甾烷醇甾体皂苷。其中，8种化合物通过与相应标准品比较得到明确鉴定。该结果为阐明麦冬活性物质基础提供了全面的见解和指导。
