Department of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
Molecules. 2018 Dec 6;23(12):3222. doi: 10.3390/molecules23123222.
is used in traditional Chinese medicine and has functions of clearing heat and drying dampness, as well as purging liver and gallbladder fire. A highly sensitive and effective strategy for rapid screening and identification of target constituents has been developed by using ultra high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap) in crude and wine-processed . Based on the accurate mass measurement (<5 ppm), retention times, and MS fragmentation ions, 52 constituents were unambiguously or tentatively characterized from , including 21 iridoids, 11 flavonoids, 19 xanthones, and a triterpenoid. This study demonstrated that the established method could be a rapid, effective analytical tool for screening and characterization of compounds in the complex systems of . By comparing the structure and peak areas of chemical constituents in crude and wine-processed , we found that some compounds in crude and wine-processed were significantly different.
被广泛应用于传统中医药，具有清热燥湿、清肝利胆泻火的功效。采用超高效液相色谱-线性离子阱-Orbitrap 质谱联用（UHPLC-LTQ-Orbitrap）建立了一种快速筛选和鉴定目标成分的高灵敏度和高效策略。基于精确质量测量（<5 ppm）、保留时间和 MS 碎片离子，从中 unambiguously 或tentatively 鉴定出 52 种成分，包括 21 种环烯醚萜类、11 种黄酮类、19 种二氢黄酮类和一种三萜类。该研究表明，所建立的方法可以成为一种快速、有效的分析工具，用于筛选和鉴定复杂体系中的化合物。通过比较和酒炮制前后化学成分的结构和峰面积，发现和酒炮制前后的化学成分有明显差异。
