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揭示硫磺熏制中药材的化学成分：三重合成方法结合 UHPLC-LTQ-Orbitrap MS——以麦冬甾体皂苷为例。

Unveiling the Chemical Composition of Sulfur-Fumigated Herbs: A Triple Synthesis Approach Using UHPLC-LTQ-Orbitrap MS-A Case Study on Steroidal Saponins in Ophiopogonis Radix.

机构信息

School of Traditional Chinese Medicine, Binzhou Medical University, Yantai 264003, China.

School of Pharmacy, Binzhou Medical University, Yantai 264003, China.

出版信息

Molecules. 2024 Feb 2;29(3):702. doi: 10.3390/molecules29030702.

DOI:10.3390/molecules29030702
PMID:38338446
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10856428/
Abstract

Ophiopogonis Radix (OR) is a traditional Chinese medicine. In recent years, in order to achieve the purpose of drying, bleaching, sterilizing and being antiseptic, improving appearance, and easy storage, people often use sulfur fumigation for its processing. However, changes in the chemical composition of medicinal herbs caused by sulfur fumigation can lead to the transformation and loss of potent substances. Therefore, the development of methods to rapidly reveal the chemical transformation of medicinal herbs induced by sulfur fumigation can guarantee the safe clinical use of medicines. In this study, a combined full scan-parent ions list-dynamic exclusion acquisition-diagnostic product ions analysis strategy based on UHPLC-LTQ-Orbitrap MS was proposed for the analysis of steroidal saponins and their transformed components in sulfur-fumigated Ophiopogonis Radix (SF-OR). Based on precise mass measurements, chromatographic behavior, neutral loss ions, and diagnostic product ions, 286 constituents were screened and identified from SF-OR, including 191 steroidal saponins and 95 sulfur-containing derivatives (sulfates or sulfites). The results indicated that the established strategy was a valuable and effective analytical tool for comprehensively characterizing the material basis of SF-OR, and also provided a basis for potential chemical changes in other sulfur-fumigated herbs.

摘要

麦冬是一种传统中药。近年来，为了达到干燥、漂白、消毒和防腐、改善外观和便于储存的目的，人们常对其进行硫磺熏蒸处理。然而，硫磺熏蒸会导致草药化学成分发生变化，从而导致有效物质的转化和损失。因此，开发快速揭示硫磺熏蒸诱导的草药化学转化的方法可以保证药物的安全临床使用。本研究提出了一种基于 UHPLC-LTQ-Orbitrap MS 的全扫描-母离子列表-动态排除采集-诊断产物离子分析策略，用于分析硫磺熏蒸麦冬（SF-OR）中的甾体皂苷及其转化成分。基于精确质量测量、色谱行为、中性丢失离子和诊断产物离子，从 SF-OR 中筛选和鉴定了 286 种成分，包括 191 种甾体皂苷和 95 种含硫衍生物（硫酸盐或亚硫酸盐）。结果表明，所建立的策略是一种全面表征 SF-OR 物质基础的有价值且有效的分析工具，也为其他硫磺熏蒸草药的潜在化学变化提供了依据。

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