Laboratory of General and Molecular Genetics, RI "Biology", Faculty of Biology Yerevan State University, 8, Charents Str, 0025 Yerevan, Armenia.
Scientific Center of Zoology and Hydroecology, National Academy of Sciences of Armenia, 7 Paruyr Sevak, 0014, Yerevan, Armenia.
Chemosphere. 2018 Oct;209:1-6. doi: 10.1016/j.chemosphere.2018.05.142. Epub 2018 May 24.
The clastogenic effects of water samples in seven locations of Lake Sevan (Armenia) with the application of Trad-MCN (micronuclei) bioassay using Tradescantia (clone 02) were investigated. A significant increase in the frequency of micronuclei in tetrads of pollen microspores and tetrads with micronuclei exposed to the test samples compared to the control has been revealed. A multivariate analysis indicated linkage between the frequencies of occurrence of micronuclei in the cells and Ni and Co ions. The results were compared with the endpoints of another Tradescantia-based test system (stamen hair mutation test) performed on the same water samples and generation of the plant: occurrences of micronuclei in sporogenic cells coincided with that of non-surviving stamen hair.
应用Trad-MCN（微核）生物测定法，用菘蓝（克隆 02）对塞凡湖（亚美尼亚）七个地点的水样进行了致突变作用研究。与对照组相比，暴露于测试样品的花粉小孢子四分体和含微核的四分体中的微核频率显著增加。多元分析表明，细胞中微核出现的频率与 Ni 和 Co 离子之间存在关联。将这些结果与在同一批水样和植物世代上进行的另一个基于菘蓝的测试系统（花粉母细胞突变测试）的终点进行了比较：生殖细胞中的微核出现与不育的花药毛出现相一致。
