Sayad Arezou, Hajifathali Abbas, Omrani Mir Davood, Arsang-Jang Shahram, Hamidieh Amir Ali, Taheri Mohammad
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Taleghani Bone Marrow Transplantation Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2018 Jun;17(3):274-280.
Recently, Long noncoding RNAs (lncRNAs) have been described as regulatory factors for several biological mechanisms through regulating the gene expression. Among them the TNF and HNRNPL related immunoregulatory (THRIL) lncRNA may be involved in the pathogenesis of immune-related and inflammatory disease through controlling the expression of the tumor necrosis factor-alpha (TNF-α) expression. In this case-control study, we investigate the THRIL expression in blood 25 samples of de novo acute myeloid leukemia (AML) cases (10 females and 15 males, mean age±SD: 35.1±3.2 years) in comparison to 50 healthy age and sex matched controls (21 females and 29 males, mean age±SD: 34.9± 3.1) using real-time quantitative reverse transcription-PCR (qRT-PCR) in order to explore any association between THRIL and AML. Our results revealed that there was no significant difference in the expression level of THRIL lncRNA between AML patients and healthy individuals (p=0.2, 95% CI=-0.129-28.35). In addition, there was no significant association between male subgroup and THRIL expression as well as females (p=0.08, 95% CI=-0.197-19.251, p=0.4, 95% CI=-0.185-12.041, respectively). In comparison between control group and FAB classification subtypes of AML patients, there was not any significant association. In conclusion, our study showed that THRIL cannot be used as an informative biomarker for AML diagnosis, however, our results need to be clarify by evolution of more cases.
最近，长链非编码RNA（lncRNAs）已被描述为通过调节基因表达参与多种生物学机制的调控因子。其中，肿瘤坏死因子和异质性核糖核蛋白L相关免疫调节（THRIL）lncRNA可能通过控制肿瘤坏死因子-α（TNF-α）的表达参与免疫相关和炎症性疾病的发病机制。在这项病例对照研究中，我们使用实时定量逆转录PCR（qRT-PCR）检测了25例初发急性髓系白血病（AML）患者（10名女性和15名男性，平均年龄±标准差：35.1±3.2岁）血液中THRIL的表达，并与50名年龄和性别匹配的健康对照者（21名女性和29名男性，平均年龄±标准差：34.9±3.1）进行比较，以探讨THRIL与AML之间的任何关联。我们的结果显示，AML患者与健康个体之间THRIL lncRNA的表达水平无显著差异（p = 0.2，95%可信区间=-0.129 - 28.35）。此外，男性亚组与THRIL表达之间以及女性亚组与THRIL表达之间均无显著关联（分别为p = 0.08，95%可信区间=-0.197 - 19.251；p = 0.4，95%可信区间=-0.185 - 12.041）。在对照组与AML患者的FAB分类亚型之间进行比较，未发现任何显著关联。总之，我们的研究表明THRIL不能用作AML诊断的信息性生物标志物，然而，我们的结果需要通过更多病例的研究来进一步阐明。
