Department of Medical Biochemistry and Molecular Biology, Fayoum University, Al Fayoum, Egypt.
Department of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University, Al Fayoum, Egypt.
IUBMB Life. 2020 Sep;72(9):1941-1950. doi: 10.1002/iub.2310. Epub 2020 Jun 20.
BACKGROUND/AIMS: Pediatric immune thrombocytopenia (ITP) is an autoimmune disease; whose etiology is not exactly understood and seems to be highly multifactorial. Long non-coding RNAs (lncRNAs) are key regulators of different actions, which contribute to the development of many autoimmune diseases. To gain a further understanding, we estimated the relative expression of lncRNAs Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and tumor necrosis factor-α (TNF-α) and heterogeneous nuclear ribonucleoprotein L (hnRNPL) immune-regulatory lncRNA (THRIL) in pediatric ITP.
In this case-control study, analysis of the expression profiles of these lncRNAs in blood samples from children with ITP and healthy controls (HCs) using quantitative real-time PCR was done. The association of MALAT1 and THRIL with ITP clinical features and their potential usage as non-invasive circulating biomarkers for ITP diagnosis was also evaluated. The receiver operating characteristic curve was constructed, and an area under the curve was analyzed.
Both lncRNAs MALAT1 and THRIL were significantly upregulated in ITP patients in comparison to HCs ( p < .0001 and = .001 respectively). In addition, there was a positive significant correlation between the expression level of both biomarkers among patients (r = 0.745, p < .0001). At cutoff points of 1.17 and 1.27 for lncRNAs MALAT1and THRIL, respectively, both biomarkers had an excellent specificity (100% for both) and fair sensitivity (63.6 and 73.3% for lncRNAs MALAT1and THRIL, respectively). Improvement of biomarkers specificity was obtained by evaluation of the combined expression of both biomarkers. Serum lncRNAs MALAT1 and THRIL could be used as potential biomarkers in differentiating childhood ITP patients and HCs.
背景/目的：小儿免疫性血小板减少症（ITP）是一种自身免疫性疾病；其病因尚不完全清楚，似乎高度多因素。长非编码 RNA（lncRNA）是不同作用的关键调节因子，有助于许多自身免疫性疾病的发展。为了进一步了解，我们估计了小儿 ITP 中 lncRNA 转移相关肺腺癌转录本 1（MALAT1）和肿瘤坏死因子-α（TNF-α）和异质核核糖核蛋白 L（hnRNPL）免疫调节 lncRNA（THRIL）的相对表达。
在这项病例对照研究中，通过定量实时 PCR 分析了这些 lncRNA 在 ITP 患儿和健康对照（HC）血液样本中的表达谱。还评估了 MALAT1 和 THRIL 与 ITP 临床特征的关联及其作为 ITP 诊断的非侵入性循环生物标志物的潜在用途。构建了接收者操作特征曲线，并分析了曲线下面积。
与 HCs 相比，ITP 患者的 lncRNA MALAT1 和 THRIL 均显著上调（p <.0001 和=.001）。此外，患者中两种生物标志物的表达水平之间存在显著正相关（r = 0.745，p <.0001）。在 lncRNA MALAT1 和 THRIL 的截止点分别为 1.17 和 1.27 时，两种生物标志物均具有出色的特异性（均为 100%）和中等的敏感性（lncRNA MALAT1 和 THRIL 的敏感性分别为 63.6%和 73.3%）。通过评估两种生物标志物的联合表达，可以提高生物标志物的特异性。血清 lncRNA MALAT1 和 THRIL 可作为鉴别小儿 ITP 患者和 HCs 的潜在生物标志物。
