Department of Veterinary Biomedical Sciences, Laboratory of Integrative Neuroendocrinology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada.
Department of Veterinary Biomedical Sciences, Laboratory of Integrative Neuroendocrinology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada.
Domest Anim Endocrinol. 2018 Oct;65:56-66. doi: 10.1016/j.domaniend.2018.04.006. Epub 2018 May 9.
Nesfatin-1 is a naturally occurring 82-amino acid protein encoded in the precursor nucleobindin-2 (NUCB2) and has been implicated in multiple physiological functions, including food intake and blood glucose regulation. This study aimed to characterize nesfatin-1 in domestic species, especially cats (Felis catus), dogs (Canis lupus familiaris), and pigs (Sus scrofa). Our in silico analysis demonstrated that the NUCB2/nesfatin-1 amino acid sequence, especially the bioactive core region of the peptide, is very highly conserved (more than 90% identity) in domestic animals. Expression of mRNAs encoding NUCB2/nesfatin-1 was detected in the cat, dog, and pig stomach and pancreas. Immunohistochemistry revealed the presence of nesfatin-1 in the gastric mucosa of the stomach of dogs, cats, and pigs, and in the pancreatic islet β-cells of dogs and pigs. No nesfatin-1 immunoreactivity was found in the cat pancreas. Nesfatin-1 was detected in the serum of dog, cat, pig, bison, cow, horse, sheep, and chicken. Circulating nesfatin-1 in male and female dogs remained unchanged at 60 min after glucose administration, suggesting a lack of meal responsiveness in nesfatin-1 secretion in this species. The presence of nesfatin-1 in the gastric and endocrine pancreatic tissues suggests possible roles for this peptide in the metabolism of domestic animals. Future research should focus on elucidating the species-specific functions and mechanisms of action of nesfatin-1 in health and disease of domestic animals.
内脂素-1 是一种天然存在的 82 个氨基酸蛋白，由前体核结合蛋白 2（NUCB2）编码，与多种生理功能有关，包括摄食和血糖调节。本研究旨在研究内脂素-1 在包括猫（Felis catus）、狗（Canis lupus familiaris）和猪（Sus scrofa）在内的家畜中的特征。我们的计算机分析表明，NUCB2/内脂素-1 氨基酸序列，特别是肽的生物活性核心区域，在家畜中高度保守（超过 90%的同一性）。在猫、狗和猪的胃和胰腺中检测到编码 NUCB2/内脂素-1 的 mRNA 的表达。免疫组织化学显示，狗、猫和猪的胃黏膜中存在内脂素-1，狗和猪的胰岛β细胞中也存在内脂素-1。猫的胰腺中未发现内脂素-1 免疫反应性。狗、猫、猪、野牛、牛、马、绵羊和鸡的血清中检测到内脂素-1。在葡萄糖给药后 60 分钟，雄性和雌性狗的循环内脂素-1保持不变，表明该物种的内脂素-1分泌对进食没有反应。内脂素-1 存在于胃和内分泌胰腺组织中，提示该肽可能在家畜的代谢中发挥作用。未来的研究应集中阐明内脂素-1在健康和疾病中的种特异性功能和作用机制。
