College of Resources and Environment, Southwest University, Chongqing 400716, China.
College of Resources and Environment, Southwest University, Chongqing 400716, China.
Ecotoxicol Environ Saf. 2018 Oct;161:474-481. doi: 10.1016/j.ecoenv.2018.06.030. Epub 2018 Jun 14.
Through a 60-day microcosm incubation, the effect of 3, 4-dimethylpyrazole phosphate (DMPP) on the activities and abundances of ammonia-oxidizers and denitrifiers in phenanthrene-polluted soil was investigated. Five treatments were conducted for clean soil (CK), phenanthrene added (P), phenanthrene and DMPP added (PD), phenanthrene and urea added (PU), and phenanthrene, urea, and DMPP added (PUD) soils. The results indicate that the potential nitrification rate (PNR) in the P treatment was significantly higher than that in the PD treatment only on day 7, whereas the PNR in the PU treatment was significantly higher than that in the PUD treatment on each sampling day. The abundance of soil ammonia-oxidizing bacteria (AOB) in the PU treatment was significantly higher than that in the PUD treatment on each sampling day. Moreover, the abundance of AOB but rather than the ammonia-oxidizing archaea (AOA) had significantly positive correlation with soil PNR (P < 0.05). DMPP showed no obvious effect on the soil denitrification enzyme activity (DEA), which could have inhibited the abundances of denitrification-related narG, nirS, and nirK genes. The results of this study should provide a deeper understanding of the interaction between soil polycyclic aromatic hydrocarbons (PAH) contamination, ammonia oxidization, and denitrification, and offer valuable information for assessing the potential contribution of denitrification for soil PAH elimination.
通过 60 天的微宇宙培养，研究了 3,4-二甲基吡唑磷酸盐（DMPP）对菲污染土壤中氨氧化菌和反硝化菌活性和丰度的影响。共设置了 5 种处理，分别为清洁土壤（CK）、添加菲（P）、添加菲和 DMPP（PD）、添加菲和尿素（PU）以及添加菲、尿素和 DMPP（PUD）的土壤。结果表明，仅在第 7 天，添加菲的处理（P）的潜在硝化速率（PNR）明显高于添加菲和 DMPP 的处理（PD），而添加尿素的处理（PU）的 PNR 在每个采样日均明显高于添加菲、尿素和 DMPP 的处理（PUD）。在每个采样日，添加尿素的处理（PU）中土壤氨氧化细菌（AOB）的丰度均明显高于添加菲、尿素和 DMPP 的处理（PUD）。此外，AOB 的丰度与土壤 PNR 呈显著正相关（P<0.05），而 AOA 则没有。DMPP 对土壤反硝化酶活性（DEA）没有明显影响，但可能抑制了与反硝化相关的 narG、nirS 和 nirK 基因的丰度。本研究结果应能更深入地了解土壤多环芳烃（PAH）污染、氨氧化和反硝化之间的相互作用，并为评估反硝化对土壤 PAH 去除的潜在贡献提供有价值的信息。
