Helmholtz Zentrum München, German Research Center for Environmental Health, Department of Terrestrial Ecogenetics, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany.
Chemosphere. 2011 Jun;84(1):182-6. doi: 10.1016/j.chemosphere.2011.02.086. Epub 2011 Mar 23.
In agricultural plant production nitrification inhibitors like 3,4-dimethylpyrazole phosphate (DMPP) are used to retard the microbial nitrification process of fertilized ammonium to enhance the nitrogen supply for cultivated crops and to reduce nitrogen losses from the production system. Besides the well-known ammonia-oxidizing bacteria (AOB) it is known for a few years that also ammonia-oxidizing archaea (AOA) are able to perform the first step in nitrification, hence being also a target for a nitrification inhibitor. However, so far no information are available concerning the effectiveness of DMPP and its extent towards AOB and AOA, neither in bulk soil nor in the root-rhizosphere complex. We investigated in a field experiment performed according to agricultural practice the effect of DMPP on the abundance of AOB and AOA two, four and eight weeks after fertilization. We observed impaired abundances of AOB but not of AOA in both soil compartments that were still visible eight weeks after application, possibly indicating a reduced effectiveness of the nitrification inhibitor in our study.
在农业植物生产中，硝化抑制剂如 3,4-二甲基吡唑磷酸盐（DMPP）被用于延缓施肥铵的微生物硝化过程，以增强栽培作物的氮供应，并减少生产系统中的氮损失。除了众所周知的氨氧化细菌（AOB）外，近年来还已知氨氧化古菌（AOA）能够进行硝化作用的第一步，因此也是硝化抑制剂的靶标。然而，迄今为止，关于 DMPP 在土壤和根-根际复合体中对 AOB 和 AOA 的有效性及其程度，尚无任何信息。我们根据农业实践进行了田间试验，研究了 DMPP 对施肥后两、四和八周时 AOB 和 AOA 丰度的影响。我们观察到在两个土壤隔室中 AOB 的丰度受到了抑制，但 AOA 却没有受到抑制，这在施用八周后仍然可见，这可能表明在我们的研究中，硝化抑制剂的效果降低了。
